Characterization of Trichomonas vaginalis haemolysis

D. C.Dai Ley, Te Hung Chang, J. F. Alderete

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64 Scopus citations

Abstract

The haemolytic activity of live Trichomonas vaginalis organisms was investigated. Optimal haemolysis of human erythrocytes was observed at a parasite to erythrocyte ratio of 1:5 during a 2 h incubation period. No haemolytic activity was detected in concentrated culture supernatants after overnight growth of trichomonads or when parasites were separated from erythrocytes by a 3/im filter, suggesting a contact-dependent mechanism for haemolysis. The haemolytic activity was temperature-dependent and maximal haemolysis occurred at 37 °C. Treatment of trichomonads with metronidazole reduced levels of haemolysis by >50%. Maximal haemolysis occurred at the pH range of the vagina during trichomoniasis. iV-a-tosyl-L-lysyl-chloromethyl ketone and iodoacetamide, inhibitors of trichomonad cysteine proteinases, reduced the haemolytic activity of live parasites.

Original languageEnglish (US)
Pages (from-to)171-175
Number of pages5
JournalParasitology
Volume101
Issue number2
DOIs
StatePublished - Oct 1990

Keywords

  • cysteine proteinases
  • haemolytic activity
  • metronidazole
  • Trichomonas vaginalis

ASJC Scopus subject areas

  • Parasitology
  • Animal Science and Zoology
  • Infectious Diseases

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