Characterization of transgenic mice that overexpress both copper zinc superoxide dismutase and catalase

James Mele, Holly Van Remmen, Jan Vijg, Arlan Richardson

Research output: Contribution to journalReview article

38 Scopus citations

Abstract

Transgenic mice overexpressing both Cu/ZnSOD and catalase [Tg(SOD1/CAT)+/0] were used to evaluate the effects of overexpression of both genes against oxidative stress. Characterization of these transgenic mice revealed that catalase or Cu/ZnSOD activities were two- to fourfold higher in the tissues of transgenic mice compared to wild-type mice, and the activities of the other major antioxidant enzymes were not altered in the tissues of the transgenic mice. The murine embryonic fibroblasts (MEFs) from the Tg(SOD1/CAT)+/0 and MEFs overexpressing Cu/ZnSOD were more resistant to paraquat cytotoxicity, relative to wild-type MEFs. The MEFs from Tg(SOD1/CAT)+/0 tended to be more resistant (up to 2.25-fold) to paraquat cytotoxicity than MEFs overexpressing either Cu/ZnSOD or catalase alone. MEFs from Tg(CAT)+/0 and Tg(SOD1/CAT)+/0 were equally as resistant to hydrogen peroxide cytotoxicity. However, there were no significant differences in whole animal survival against either paraquat or γ-radiation.

Original languageEnglish (US)
Pages (from-to)628-638
Number of pages11
JournalAntioxidants and Redox Signaling
Volume8
Issue number3-4
DOIs
StatePublished - Mar 2006

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology

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