Characterization of the promoter region of the viral interferon regulatory factor encoded by Kaposi's sarcoma-associated herpesvirus

Xin Ping Wang; Yan Jin Zhang; Jian Hong Deng; Hong Yi Pan; Fu Chun Zhou; Eduardo A. Montalvo; Shou Jiang Gao

doi:10.1038/sj.onc.1204115

Characterization of the promoter region of the viral interferon regulatory factor encoded by Kaposi's sarcoma-associated herpesvirus

Xin Ping Wang, Yan Jin Zhang, Jian Hong Deng, Hong Yi Pan, Fu Chun Zhou, Eduardo A. Montalvo, Shou Jiang Gao

Department of Cell Systems & Anatomy

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

Viral interferon regulatory factor (vIRF) encoded by Kaposi's sarcoma-associated herpesvirus (KSHV) inhibits the expression of interferon-responsive genes, causes cellular transformation and transactivates KSHV genes. In the present study, we characterized the mRNA expression pattern of the vIRF gene and its promoter. A vIRF transcript of 1.7 kb in size was detected in low level in uninduced KSHV-infected cells and its expression was inducible by 12-O-tetradecanoylphorbol-13acetate (TPA), sensitive to cycloheximide and resistant to phosphonoacetic acid. The transcription start site was mapped to 79 nt upstream of the ATG initiation site by 5′-RACE. Mutagenesis analysis identified a region between -56 and the transcription start site (+1) as the minimal promoter region that contains a functional TATA box at -27. A region between -337 and -125 contains a repressor domain negated by sequence from -991 to -499 in BCBL-1 cells, a region which was also identified to be responsive to TPA induction. These results demonstrate vlRF as a KSHV early gene, identify its promoter and define the promoter regions that contain regulatory elements controlling vIRF transcription.

Original language	English (US)
Pages (from-to)	523-530
Number of pages	8
Journal	Oncogene
Volume	20
Issue number	4
DOIs	https://doi.org/10.1038/sj.onc.1204115
State	Published - Jan 25 2001

Keywords

Kaposi's sarcoma-associated herpesvirus
Oncogene
Promoter
TPA induction
Viral interferon regulatory factor

ASJC Scopus subject areas

Molecular Biology
Genetics
Cancer Research

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10.1038/sj.onc.1204115

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title = "Characterization of the promoter region of the viral interferon regulatory factor encoded by Kaposi's sarcoma-associated herpesvirus",

abstract = "Viral interferon regulatory factor (vIRF) encoded by Kaposi's sarcoma-associated herpesvirus (KSHV) inhibits the expression of interferon-responsive genes, causes cellular transformation and transactivates KSHV genes. In the present study, we characterized the mRNA expression pattern of the vIRF gene and its promoter. A vIRF transcript of 1.7 kb in size was detected in low level in uninduced KSHV-infected cells and its expression was inducible by 12-O-tetradecanoylphorbol-13acetate (TPA), sensitive to cycloheximide and resistant to phosphonoacetic acid. The transcription start site was mapped to 79 nt upstream of the ATG initiation site by 5′-RACE. Mutagenesis analysis identified a region between -56 and the transcription start site (+1) as the minimal promoter region that contains a functional TATA box at -27. A region between -337 and -125 contains a repressor domain negated by sequence from -991 to -499 in BCBL-1 cells, a region which was also identified to be responsive to TPA induction. These results demonstrate vlRF as a KSHV early gene, identify its promoter and define the promoter regions that contain regulatory elements controlling vIRF transcription.",

keywords = "Kaposi's sarcoma-associated herpesvirus, Oncogene, Promoter, TPA induction, Viral interferon regulatory factor",

author = "Wang, {Xin Ping} and Zhang, {Yan Jin} and Deng, {Jian Hong} and Pan, {Hong Yi} and Zhou, {Fu Chun} and Montalvo, {Eduardo A.} and Gao, {Shou Jiang}",

note = "Funding Information: This work is supported by research grants to SJ Gao from the Howard Hughes Medical Institute through the University of Texas Health Science Center at San Antonio for the Research Resource Program for Medical Schools S/G 0#7, Elsa U Pardee Foundation, Association for International Cancer Research, and National Institute of Health (HL60604-01).",

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doi = "10.1038/sj.onc.1204115",

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T1 - Characterization of the promoter region of the viral interferon regulatory factor encoded by Kaposi's sarcoma-associated herpesvirus

AU - Wang, Xin Ping

AU - Zhang, Yan Jin

AU - Deng, Jian Hong

AU - Pan, Hong Yi

AU - Zhou, Fu Chun

AU - Montalvo, Eduardo A.

AU - Gao, Shou Jiang

N1 - Funding Information: This work is supported by research grants to SJ Gao from the Howard Hughes Medical Institute through the University of Texas Health Science Center at San Antonio for the Research Resource Program for Medical Schools S/G 0#7, Elsa U Pardee Foundation, Association for International Cancer Research, and National Institute of Health (HL60604-01).

PY - 2001/1/25

Y1 - 2001/1/25

N2 - Viral interferon regulatory factor (vIRF) encoded by Kaposi's sarcoma-associated herpesvirus (KSHV) inhibits the expression of interferon-responsive genes, causes cellular transformation and transactivates KSHV genes. In the present study, we characterized the mRNA expression pattern of the vIRF gene and its promoter. A vIRF transcript of 1.7 kb in size was detected in low level in uninduced KSHV-infected cells and its expression was inducible by 12-O-tetradecanoylphorbol-13acetate (TPA), sensitive to cycloheximide and resistant to phosphonoacetic acid. The transcription start site was mapped to 79 nt upstream of the ATG initiation site by 5′-RACE. Mutagenesis analysis identified a region between -56 and the transcription start site (+1) as the minimal promoter region that contains a functional TATA box at -27. A region between -337 and -125 contains a repressor domain negated by sequence from -991 to -499 in BCBL-1 cells, a region which was also identified to be responsive to TPA induction. These results demonstrate vlRF as a KSHV early gene, identify its promoter and define the promoter regions that contain regulatory elements controlling vIRF transcription.

AB - Viral interferon regulatory factor (vIRF) encoded by Kaposi's sarcoma-associated herpesvirus (KSHV) inhibits the expression of interferon-responsive genes, causes cellular transformation and transactivates KSHV genes. In the present study, we characterized the mRNA expression pattern of the vIRF gene and its promoter. A vIRF transcript of 1.7 kb in size was detected in low level in uninduced KSHV-infected cells and its expression was inducible by 12-O-tetradecanoylphorbol-13acetate (TPA), sensitive to cycloheximide and resistant to phosphonoacetic acid. The transcription start site was mapped to 79 nt upstream of the ATG initiation site by 5′-RACE. Mutagenesis analysis identified a region between -56 and the transcription start site (+1) as the minimal promoter region that contains a functional TATA box at -27. A region between -337 and -125 contains a repressor domain negated by sequence from -991 to -499 in BCBL-1 cells, a region which was also identified to be responsive to TPA induction. These results demonstrate vlRF as a KSHV early gene, identify its promoter and define the promoter regions that contain regulatory elements controlling vIRF transcription.

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Characterization of the promoter region of the viral interferon regulatory factor encoded by Kaposi's sarcoma-associated herpesvirus

Abstract

Keywords

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