This unit describes an in vitro assay for characterization of the picrotoxin site of GABAA receptors in rat brain membranes using various radioligands.Methods and representative data for Scatchard analysis (Kd, Bmax determination), association kinetics, dissociation kinetics, and competition assays (IC50, Ki determination) are included.
|Original language||English (US)|
|Journal||Current Protocols in Pharmacology|
|State||Published - 2013|
- Allosteric regulation
- Picrotoxin binding site
ASJC Scopus subject areas