Characterization of the picrotoxin site of GABAA receptors

Ashok K. Mehta; Maharaj K. Ticku

doi:10.1002/0471141755.ph0118s63

Characterization of the picrotoxin site of GABA_A receptors

Ashok K. Mehta, Maharaj K. Ticku

Department of Pharmacology

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

This unit describes an in vitro assay for characterization of the picrotoxin site of GABA_A receptors in rat brain membranes using various radioligands.Methods and representative data for Scatchard analysis (K_d, B_max determination), association kinetics, dissociation kinetics, and competition assays (IC₅₀, K_i determination) are included.

Original language	English (US)
Article number	1.18
Journal	Current Protocols in Pharmacology
Volume	1
Issue number	SPPL.63
DOIs	https://doi.org/10.1002/0471141755.ph0118s63
State	Published - 2013

Keywords

Allosteric regulation
Convulsants
Picrotoxin binding site

ASJC Scopus subject areas

Pharmacology

Access to Document

10.1002/0471141755.ph0118s63

Cite this

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abstract = "This unit describes an in vitro assay for characterization of the picrotoxin site of GABAA receptors in rat brain membranes using various radioligands.Methods and representative data for Scatchard analysis (Kd, Bmax determination), association kinetics, dissociation kinetics, and competition assays (IC50, Ki determination) are included.",

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