Characterization of the picrotoxin site of GABAA receptors

Ashok K. Mehta, Maharaj K. Ticku

Research output: Contribution to journalArticlepeer-review

4 Scopus citations


This unit describes an in vitro assay for characterization of the picrotoxin site of GABAA receptors in rat brain membranes using various radioligands.Methods and representative data for Scatchard analysis (Kd, Bmax determination), association kinetics, dissociation kinetics, and competition assays (IC50, Ki determination) are included.

Original languageEnglish (US)
Article number1.18
JournalCurrent Protocols in Pharmacology
Issue numberSPPL.63
StatePublished - 2013


  • Allosteric regulation
  • Convulsants
  • Picrotoxin binding site

ASJC Scopus subject areas

  • Pharmacology


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