Characterization of the mouse tartrate-resistant acid phosphatase (TRAP) gene promoter

S. V. Reddy, J. E. Hundley, J. J. Windle, O. Alcantara, R. Linn, Robin J Leach, D. H. Boldt, G. D. Roodman

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

Tartrate-resistant acid phosphatase (TRAP) is an iron-binding protein that is highly expressed in osteoclasts. To characterize the regulation of TRAP gene expression, progressive 5' and 3' deletions of a 1.8 kb fragment containing the 5'-flanking sequence were fused to a luciferase reporter gene. Two nonoverlapping regions of this 1.8 kb fragment had promoter activity. The upstream promoter (P1) was located within the region from -881 bp to -463 bp relative to the ATG, while the downstream promoter (P2) was located between -363 bp to -1 bp in a region we have previously shown to be an intron in transcripts originating from the upstream promoter. A putative repressor region for the P2 promoter at -1846 bp to -1240 bp and a putative enhancer region at -962 bp to -881 bp relative to the ATG were identified. PCR analysis of promoter-specific transcription of the TRAP gene in various murine tissues showed that both promoters were active in several tissues. Transferrin-bound iron increased P1 promoter activity 2.5-fold and heroin decreased P1 promoter activity, but neither had any effect on P2 activity. These data show that the transcriptional regulation of the TRAP gene is complex and that iron may play a key role in TRAP gene regulation.

Original languageEnglish (US)
Pages (from-to)601-606
Number of pages6
JournalJournal of Bone and Mineral Research
Volume10
Issue number4
StatePublished - 1995
Externally publishedYes

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Genes
Iron
Iron-Binding Proteins
5' Flanking Region
Heroin
Osteoclasts
Transferrin
Luciferases
Reporter Genes
Genetic Promoter Regions
Introns
Tartrate-Resistant Acid Phosphatase
Gene Expression
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Surgery

Cite this

Reddy, S. V., Hundley, J. E., Windle, J. J., Alcantara, O., Linn, R., Leach, R. J., ... Roodman, G. D. (1995). Characterization of the mouse tartrate-resistant acid phosphatase (TRAP) gene promoter. Journal of Bone and Mineral Research, 10(4), 601-606.

Characterization of the mouse tartrate-resistant acid phosphatase (TRAP) gene promoter. / Reddy, S. V.; Hundley, J. E.; Windle, J. J.; Alcantara, O.; Linn, R.; Leach, Robin J; Boldt, D. H.; Roodman, G. D.

In: Journal of Bone and Mineral Research, Vol. 10, No. 4, 1995, p. 601-606.

Research output: Contribution to journalArticle

Reddy, SV, Hundley, JE, Windle, JJ, Alcantara, O, Linn, R, Leach, RJ, Boldt, DH & Roodman, GD 1995, 'Characterization of the mouse tartrate-resistant acid phosphatase (TRAP) gene promoter', Journal of Bone and Mineral Research, vol. 10, no. 4, pp. 601-606.
Reddy, S. V. ; Hundley, J. E. ; Windle, J. J. ; Alcantara, O. ; Linn, R. ; Leach, Robin J ; Boldt, D. H. ; Roodman, G. D. / Characterization of the mouse tartrate-resistant acid phosphatase (TRAP) gene promoter. In: Journal of Bone and Mineral Research. 1995 ; Vol. 10, No. 4. pp. 601-606.
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