Characterization of the mouse JAB1 cDNA and protein

Mangkey A. Bounpheng, Irena N. Melnikova, Sherry G. Dodds, Heping Chen, Neal G. Copeland, Debra J. Gilbert, Nancy A. Jenkins, Barbara A. Christy

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

JAB1 was originally described as a transcriptional coactivator of c-Jun and Jun D. Recent data suggests that JAB1 is a component of a large protein complex, the JAB1 signalosome in mammals and the COP9 complex in plants. The JAB1 signalosome is implicated in the phosphorylation of selected transcription factors, while the COP9 complex is involved in repression of photomorphogenesis in Arabidopsis. In this study, we describe the partial characterization of mouse JAB1 (mJAB1). The murine JAB1 protein is encoded by a gene located on mouse chromosome 1. mJAB1 mRNA is abundantly expressed in a variety of adult tissues as well as in mouse embryos. The JAB1 protein was readily detectable in many cell types and localized to both the nucleus and cytoplasm. Endogenous JAB1 protein is relatively stable and its degradation is not perturbed by blocking 26S proteasome activity, suggesting that this protein is not degraded by the ubiquitin-mediated proteolytic pathway. (C) 2000 Elsevier Science B.V. All rights reserved.

Original languageEnglish (US)
Pages (from-to)41-50
Number of pages10
JournalGene
Volume242
Issue number1-2
DOIs
StatePublished - Jan 25 2000

Fingerprint

Complementary DNA
Proteins
Chromosomes, Human, Pair 1
Ubiquitin
Arabidopsis
Mammals
Cytoplasm
Transcription Factors
Embryonic Structures
Phosphorylation
Messenger RNA
Genes

Keywords

  • Chromosomal location
  • mRNA
  • Proteasome
  • Protein expression
  • Subcellular location
  • Ubiquitin

ASJC Scopus subject areas

  • Genetics

Cite this

Bounpheng, M. A., Melnikova, I. N., Dodds, S. G., Chen, H., Copeland, N. G., Gilbert, D. J., ... Christy, B. A. (2000). Characterization of the mouse JAB1 cDNA and protein. Gene, 242(1-2), 41-50. https://doi.org/10.1016/S0378-1119(99)00525-9

Characterization of the mouse JAB1 cDNA and protein. / Bounpheng, Mangkey A.; Melnikova, Irena N.; Dodds, Sherry G.; Chen, Heping; Copeland, Neal G.; Gilbert, Debra J.; Jenkins, Nancy A.; Christy, Barbara A.

In: Gene, Vol. 242, No. 1-2, 25.01.2000, p. 41-50.

Research output: Contribution to journalArticle

Bounpheng, MA, Melnikova, IN, Dodds, SG, Chen, H, Copeland, NG, Gilbert, DJ, Jenkins, NA & Christy, BA 2000, 'Characterization of the mouse JAB1 cDNA and protein', Gene, vol. 242, no. 1-2, pp. 41-50. https://doi.org/10.1016/S0378-1119(99)00525-9
Bounpheng MA, Melnikova IN, Dodds SG, Chen H, Copeland NG, Gilbert DJ et al. Characterization of the mouse JAB1 cDNA and protein. Gene. 2000 Jan 25;242(1-2):41-50. https://doi.org/10.1016/S0378-1119(99)00525-9
Bounpheng, Mangkey A. ; Melnikova, Irena N. ; Dodds, Sherry G. ; Chen, Heping ; Copeland, Neal G. ; Gilbert, Debra J. ; Jenkins, Nancy A. ; Christy, Barbara A. / Characterization of the mouse JAB1 cDNA and protein. In: Gene. 2000 ; Vol. 242, No. 1-2. pp. 41-50.
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