Characterization of the mouse JAB1 cDNA and protein

Mangkey A. Bounpheng, Irena N. Melnikova, Sherry G. Dodds, Heping Chen, Neal G. Copeland, Debra J. Gilbert, Nancy A. Jenkins, Barbara A. Christy

Research output: Contribution to journalArticlepeer-review

29 Scopus citations

Abstract

JAB1 was originally described as a transcriptional coactivator of c-Jun and Jun D. Recent data suggests that JAB1 is a component of a large protein complex, the JAB1 signalosome in mammals and the COP9 complex in plants. The JAB1 signalosome is implicated in the phosphorylation of selected transcription factors, while the COP9 complex is involved in repression of photomorphogenesis in Arabidopsis. In this study, we describe the partial characterization of mouse JAB1 (mJAB1). The murine JAB1 protein is encoded by a gene located on mouse chromosome 1. mJAB1 mRNA is abundantly expressed in a variety of adult tissues as well as in mouse embryos. The JAB1 protein was readily detectable in many cell types and localized to both the nucleus and cytoplasm. Endogenous JAB1 protein is relatively stable and its degradation is not perturbed by blocking 26S proteasome activity, suggesting that this protein is not degraded by the ubiquitin-mediated proteolytic pathway. (C) 2000 Elsevier Science B.V. All rights reserved.

Original languageEnglish (US)
Pages (from-to)41-50
Number of pages10
JournalGene
Volume242
Issue number1-2
DOIs
StatePublished - Jan 25 2000

Keywords

  • Chromosomal location
  • Proteasome
  • Protein expression
  • Subcellular location
  • Ubiquitin
  • mRNA

ASJC Scopus subject areas

  • Genetics

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