Characterization of the ets oncogene family member, fli-1

M. J. Klemsz, R. A. Maki, T. Papayannopoulou, J. Moore, Robert A Hromas

Research output: Contribution to journalArticle

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Abstract

The recently cloned fli-1 gene is a member of the ets oncogene family that is preferentially expressed in hematopoietic cells. It is a target of dysregulation by Friend leukemia virus insertion and translocation in Ewing's sarcoma and neuroepithelioma. In this report, we have studied the function and regulation of both murine and human fli-1. Analysis of the human and mouse fli-1 proteins showed that fli-1 binds to specific DNA sequences highly related to m-ets-2 binding sites. Methylation protection experiments showed that fli-1 and m-ets-2 contacted the same nucleotides in two different binding sites. The fli-1 protein was shown to be a transcriptional activator in co-transfection studies. Stimulation of murine bone marrow macrophages by mediators of inflammation, such as lipopolysaccharide, phorbol 12-myristate 13-acetate, interleukin-1, and interferon-γ resulted in the reduced expression of fli-1 mRNA. fli-1 was only expressed in a defined subset of human erythroleukemia cell lines.

Original languageEnglish (US)
Pages (from-to)5769-5773
Number of pages5
JournalJournal of Biological Chemistry
Volume268
Issue number8
StatePublished - Jan 1 1993
Externally publishedYes

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Oncogenes
Binding Sites
Inflammation Mediators
Methylation
Macrophages
DNA sequences
Interleukin-1
Viruses
Friend murine leukemia virus
Interferons
Lipopolysaccharides
Leukemia, Erythroblastic, Acute
Bone
Acetates
Ewing's Sarcoma
Nucleotides
Genes
Cells
Messenger RNA
Transfection

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Klemsz, M. J., Maki, R. A., Papayannopoulou, T., Moore, J., & Hromas, R. A. (1993). Characterization of the ets oncogene family member, fli-1. Journal of Biological Chemistry, 268(8), 5769-5773.

Characterization of the ets oncogene family member, fli-1. / Klemsz, M. J.; Maki, R. A.; Papayannopoulou, T.; Moore, J.; Hromas, Robert A.

In: Journal of Biological Chemistry, Vol. 268, No. 8, 01.01.1993, p. 5769-5773.

Research output: Contribution to journalArticle

Klemsz, MJ, Maki, RA, Papayannopoulou, T, Moore, J & Hromas, RA 1993, 'Characterization of the ets oncogene family member, fli-1', Journal of Biological Chemistry, vol. 268, no. 8, pp. 5769-5773.
Klemsz MJ, Maki RA, Papayannopoulou T, Moore J, Hromas RA. Characterization of the ets oncogene family member, fli-1. Journal of Biological Chemistry. 1993 Jan 1;268(8):5769-5773.
Klemsz, M. J. ; Maki, R. A. ; Papayannopoulou, T. ; Moore, J. ; Hromas, Robert A. / Characterization of the ets oncogene family member, fli-1. In: Journal of Biological Chemistry. 1993 ; Vol. 268, No. 8. pp. 5769-5773.
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