Characterization of the antioxidant status of the heterozygous manganese superoxide dismutase knockout mouse

Holly Van Remmen, Carolina Salvador, Hong Yang, Ting Ting Huang, Charles J. Epstein, Arlan Richardson

Research output: Contribution to journalArticle

131 Scopus citations

Abstract

The antioxidant status of several tissues (liver, kidney, lung, brain, heart, muscle, stomach, and spleen) from heterozygous manganese superoxide dismutase (MnSOD) mutant mice (Sod2(-/+)) was characterized. The activity of MnSOD was decreased (30 to 80%) in all tissues examined. The levels of mRNA coding for the major antioxidant enzymes (CuZnSOD, catalase, and glutathione peroxidase) were not significantly altered in liver, kidney, heart, lung, or brain in the Sod2(-/+) mice. The activities of the enzymes were not altered in any of these tissues, with the exception of a decrease in glutathione peroxidase activity in muscle in the Sod2(-/+) mice compared to the Sod2(+/+) mice. Thus, there was no up-regulation of the activities of the major antioxidant enzymes to compensate for the decrease in MnSOD activity. Reduced glutathione levels were 30 to 50% lower in the lung, brain, and muscle of the Sod2(-/+) mice compared to the wild-type Sod2(+/+) mice. In addition, the ratio of GSH/GSSG was decreased approximately 50% in Sod2(-/+) muscle, indicating that the decrease in MnSOD activity in the Sod2(-/+) mice results in some degree of oxidative stress in this tissue.

Original languageEnglish (US)
Pages (from-to)91-97
Number of pages7
JournalArchives of Biochemistry and Biophysics
Volume363
Issue number1
DOIs
StatePublished - Mar 1 1999

Keywords

  • Antioxidant enzymes
  • Glutathione
  • Knockout mice
  • MnSOD

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

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