Characterization of six type A strains of Clostridium botulinum that contain type B toxin gene sequences

Nameer Kirma; Joseph L. Ferreira; Barbara R. Baumstark

doi:10.1016/S0378-1097(03)00911-X

Characterization of six type A strains of Clostridium botulinum that contain type B toxin gene sequences

Nameer Kirma, Joseph L. Ferreira, Barbara R. Baumstark

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

Six Clostridium botulinum isolates exhibiting type A toxicity as measured by the mouse bioassay were found to contain both type A and type B neurotoxin DNA sequences. The six strains were divided into three groups based on the DNA sequence of the type B neurotoxin gene. Members of each group exhibited 100% sequence identity over the 3876 bp type B toxin open reading frame. The type B toxin sequence of all groups differed at more than 60 positions when compared to the BGB control strain.

Original language	English (US)
Pages (from-to)	159-164
Number of pages	6
Journal	FEMS Microbiology Letters
Volume	231
Issue number	2
DOIs	https://doi.org/10.1016/S0378-1097(03)00911-X
State	Published - Feb 16 2004
Externally published	Yes

Keywords

Clostridium botulinum
Neurotoxin gene sequence
Polymerase chain reaction

ASJC Scopus subject areas

Genetics
Molecular Biology
Microbiology

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10.1016/S0378-1097(03)00911-X

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title = "Characterization of six type A strains of Clostridium botulinum that contain type B toxin gene sequences",

abstract = "Six Clostridium botulinum isolates exhibiting type A toxicity as measured by the mouse bioassay were found to contain both type A and type B neurotoxin DNA sequences. The six strains were divided into three groups based on the DNA sequence of the type B neurotoxin gene. Members of each group exhibited 100% sequence identity over the 3876 bp type B toxin open reading frame. The type B toxin sequence of all groups differed at more than 60 positions when compared to the BGB control strain.",

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AU - Baumstark, Barbara R.

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Y1 - 2004/2/16

N2 - Six Clostridium botulinum isolates exhibiting type A toxicity as measured by the mouse bioassay were found to contain both type A and type B neurotoxin DNA sequences. The six strains were divided into three groups based on the DNA sequence of the type B neurotoxin gene. Members of each group exhibited 100% sequence identity over the 3876 bp type B toxin open reading frame. The type B toxin sequence of all groups differed at more than 60 positions when compared to the BGB control strain.

AB - Six Clostridium botulinum isolates exhibiting type A toxicity as measured by the mouse bioassay were found to contain both type A and type B neurotoxin DNA sequences. The six strains were divided into three groups based on the DNA sequence of the type B neurotoxin gene. Members of each group exhibited 100% sequence identity over the 3876 bp type B toxin open reading frame. The type B toxin sequence of all groups differed at more than 60 positions when compared to the BGB control strain.

KW - Clostridium botulinum

KW - Neurotoxin gene sequence

KW - Polymerase chain reaction

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Characterization of six type A strains of Clostridium botulinum that contain type B toxin gene sequences

Abstract

Keywords

ASJC Scopus subject areas

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