Characterization of six type A strains of Clostridium botulinum that contain type B toxin gene sequences

Nameer Kirma, Joseph L. Ferreira, Barbara R. Baumstark

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

Six Clostridium botulinum isolates exhibiting type A toxicity as measured by the mouse bioassay were found to contain both type A and type B neurotoxin DNA sequences. The six strains were divided into three groups based on the DNA sequence of the type B neurotoxin gene. Members of each group exhibited 100% sequence identity over the 3876 bp type B toxin open reading frame. The type B toxin sequence of all groups differed at more than 60 positions when compared to the BGB control strain.

Original languageEnglish (US)
Pages (from-to)159-164
Number of pages6
JournalFEMS Microbiology Letters
Volume231
Issue number2
DOIs
StatePublished - Feb 16 2004

Keywords

  • Clostridium botulinum
  • Neurotoxin gene sequence
  • Polymerase chain reaction

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

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