Characterization of six type A strains of Clostridium botulinum that contain type B toxin gene sequences

Nameer B Kirma, Joseph L. Ferreira, Barbara R. Baumstark

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Six Clostridium botulinum isolates exhibiting type A toxicity as measured by the mouse bioassay were found to contain both type A and type B neurotoxin DNA sequences. The six strains were divided into three groups based on the DNA sequence of the type B neurotoxin gene. Members of each group exhibited 100% sequence identity over the 3876 bp type B toxin open reading frame. The type B toxin sequence of all groups differed at more than 60 positions when compared to the BGB control strain.

Original languageEnglish (US)
Pages (from-to)159-164
Number of pages6
JournalFEMS Microbiology Letters
Volume231
Issue number2
DOIs
StatePublished - Feb 16 2004
Externally publishedYes

Fingerprint

Neurotoxins
Clostridium botulinum
Biological Assay
Open Reading Frames
Genes
rimabotulinumtoxinB

Keywords

  • Clostridium botulinum
  • Neurotoxin gene sequence
  • Polymerase chain reaction

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Characterization of six type A strains of Clostridium botulinum that contain type B toxin gene sequences. / Kirma, Nameer B; Ferreira, Joseph L.; Baumstark, Barbara R.

In: FEMS Microbiology Letters, Vol. 231, No. 2, 16.02.2004, p. 159-164.

Research output: Contribution to journalArticle

Kirma, Nameer B ; Ferreira, Joseph L. ; Baumstark, Barbara R. / Characterization of six type A strains of Clostridium botulinum that contain type B toxin gene sequences. In: FEMS Microbiology Letters. 2004 ; Vol. 231, No. 2. pp. 159-164.
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