Characterization of SIV-specific CD4+ T-helper proliferative responses in macaques immunized with live-attenuated SIV

Marie Claire Gauduin; Rhona L. Glickman; Shabbir Ahmad; Tilahun Yilma; R. Paul Johnson

doi:10.1111/j.1600-0684.1999.tb00274.x

Characterization of SIV-specific CD4⁺ T-helper proliferative responses in macaques immunized with live-attenuated SIV

Marie Claire Gauduin, Rhona L. Glickman, Shabbir Ahmad, Tilahun Yilma, R. Paul Johnson

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

Analysis of immune responses generated by live-attenuated simian immunodeficiency virus (SIV) strains may provide clues to the mechanisms of protective immunity induced by this approach. We examined SIV-specific T-helper responses in macaques immunized with the live-attenuated SIV strains SIVmac239Δnef and SIVmac239Δ3. Optimization of the concentration and duration of antigenic stimulation resulted in the detection of relatively strong SIV-specific proliferative responses, with peak stimulation indices of up to 84. SIV-specific proliferative responses were mediated by CD4⁺ T cells and were major histocompatibility (MHC) class II restricted. Limiting dilution analysis revealed SIV-specific T-helper precursor frequencies of up to 96 per 10⁶ peripheral blood mononuclear cells (PBMC). Intracellular flow-cytometric analysis demonstrated the production of interleukin (IL)-2, interferon (IFN)-γ, RANTES and macrophage inhibitory protein-1α (MIP-1α) by T lymphocytes from SIVmac239Δnef-vaccinated animals following SIV p55 stimulation. Induction of strong SIV-specific T-helper responses by live-attenuated SIV vaccines may play a role in their ability to induce protective immunity.

Original language	English (US)
Pages (from-to)	233-241
Number of pages	9
Journal	Journal of medical primatology
Volume	28
Issue number	4-5
DOIs	https://doi.org/10.1111/j.1600-0684.1999.tb00274.x
State	Published - 1999

Keywords

AIDS vaccines
CD4 T-helper responses
Simian immunodeficiency virus

ASJC Scopus subject areas

Animal Science and Zoology
veterinary(all)

Access to Document

10.1111/j.1600-0684.1999.tb00274.x

Cite this

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title = "Characterization of SIV-specific CD4+ T-helper proliferative responses in macaques immunized with live-attenuated SIV",

abstract = "Analysis of immune responses generated by live-attenuated simian immunodeficiency virus (SIV) strains may provide clues to the mechanisms of protective immunity induced by this approach. We examined SIV-specific T-helper responses in macaques immunized with the live-attenuated SIV strains SIVmac239Δnef and SIVmac239Δ3. Optimization of the concentration and duration of antigenic stimulation resulted in the detection of relatively strong SIV-specific proliferative responses, with peak stimulation indices of up to 84. SIV-specific proliferative responses were mediated by CD4+ T cells and were major histocompatibility (MHC) class II restricted. Limiting dilution analysis revealed SIV-specific T-helper precursor frequencies of up to 96 per 106 peripheral blood mononuclear cells (PBMC). Intracellular flow-cytometric analysis demonstrated the production of interleukin (IL)-2, interferon (IFN)-γ, RANTES and macrophage inhibitory protein-1α (MIP-1α) by T lymphocytes from SIVmac239Δnef-vaccinated animals following SIV p55 stimulation. Induction of strong SIV-specific T-helper responses by live-attenuated SIV vaccines may play a role in their ability to induce protective immunity.",

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AU - Ahmad, Shabbir

AU - Yilma, Tilahun

AU - Johnson, R. Paul

PY - 1999

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AB - Analysis of immune responses generated by live-attenuated simian immunodeficiency virus (SIV) strains may provide clues to the mechanisms of protective immunity induced by this approach. We examined SIV-specific T-helper responses in macaques immunized with the live-attenuated SIV strains SIVmac239Δnef and SIVmac239Δ3. Optimization of the concentration and duration of antigenic stimulation resulted in the detection of relatively strong SIV-specific proliferative responses, with peak stimulation indices of up to 84. SIV-specific proliferative responses were mediated by CD4+ T cells and were major histocompatibility (MHC) class II restricted. Limiting dilution analysis revealed SIV-specific T-helper precursor frequencies of up to 96 per 106 peripheral blood mononuclear cells (PBMC). Intracellular flow-cytometric analysis demonstrated the production of interleukin (IL)-2, interferon (IFN)-γ, RANTES and macrophage inhibitory protein-1α (MIP-1α) by T lymphocytes from SIVmac239Δnef-vaccinated animals following SIV p55 stimulation. Induction of strong SIV-specific T-helper responses by live-attenuated SIV vaccines may play a role in their ability to induce protective immunity.

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