Abstract
Analysis of immune responses generated by live-attenuated simian immunodeficiency virus (SIV) strains may provide clues to the mechanisms of protective immunity induced by this approach. We examined SIV-specific T-helper responses in macaques immunized with the live-attenuated SIV strains SIVmac239Δnef and SIVmac239Δ3. Optimization of the concentration and duration of antigenic stimulation resulted in the detection of relatively strong SIV-specific proliferative responses, with peak stimulation indices of up to 84. SIV-specific proliferative responses were mediated by CD4+ T cells and were major histocompatibility (MHC) class II restricted. Limiting dilution analysis revealed SIV-specific T-helper precursor frequencies of up to 96 per 106 peripheral blood mononuclear cells (PBMC). Intracellular flow-cytometric analysis demonstrated the production of interleukin (IL)-2, interferon (IFN)-γ, RANTES and macrophage inhibitory protein-1α (MIP-1α) by T lymphocytes from SIVmac239Δnef-vaccinated animals following SIV p55 stimulation. Induction of strong SIV-specific T-helper responses by live-attenuated SIV vaccines may play a role in their ability to induce protective immunity.
Original language | English (US) |
---|---|
Pages (from-to) | 233-241 |
Number of pages | 9 |
Journal | Journal of medical primatology |
Volume | 28 |
Issue number | 4-5 |
DOIs | |
State | Published - 1999 |
Keywords
- AIDS vaccines
- CD4 T-helper responses
- Simian immunodeficiency virus
ASJC Scopus subject areas
- Animal Science and Zoology
- veterinary(all)