Characterization of SIV-specific CD4+ T-helper proliferative responses in macaques immunized with live-attenuated SIV

Marie Claire Gauduin, Rhona L. Glickman, Shabbir Ahmad, Tilahun Yilma, R. Paul Johnson

    Research output: Contribution to journalArticle

    16 Scopus citations

    Abstract

    Analysis of immune responses generated by live-attenuated simian immunodeficiency virus (SIV) strains may provide clues to the mechanisms of protective immunity induced by this approach. We examined SIV-specific T-helper responses in macaques immunized with the live-attenuated SIV strains SIVmac239Δnef and SIVmac239Δ3. Optimization of the concentration and duration of antigenic stimulation resulted in the detection of relatively strong SIV-specific proliferative responses, with peak stimulation indices of up to 84. SIV-specific proliferative responses were mediated by CD4+ T cells and were major histocompatibility (MHC) class II restricted. Limiting dilution analysis revealed SIV-specific T-helper precursor frequencies of up to 96 per 106 peripheral blood mononuclear cells (PBMC). Intracellular flow-cytometric analysis demonstrated the production of interleukin (IL)-2, interferon (IFN)-γ, RANTES and macrophage inhibitory protein-1α (MIP-1α) by T lymphocytes from SIVmac239Δnef-vaccinated animals following SIV p55 stimulation. Induction of strong SIV-specific T-helper responses by live-attenuated SIV vaccines may play a role in their ability to induce protective immunity.

    Original languageEnglish (US)
    Pages (from-to)233-241
    Number of pages9
    JournalJournal of medical primatology
    Volume28
    Issue number4-5
    DOIs
    StatePublished - 1999

    Keywords

    • AIDS vaccines
    • CD4 T-helper responses
    • Simian immunodeficiency virus

    ASJC Scopus subject areas

    • Animal Science and Zoology
    • veterinary(all)

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