Characterization of reversible associations by sedimentation velocity with ultrascan

Borries Demeler, Emre Brookes, Renjing Wang, Virgil Schirf, Chongwoo A. Kim

Research output: Contribution to journalArticlepeer-review

36 Scopus citations

Abstract

We compare here the utility of sedimentation velocity (SV) to sedimentation equilibrium (SE) analysis for the characterization of reversible systems. Genetic algorithm optimization in UltraScan is used to optimize the model and to obtain solution properties of all components present in the system. We apply our method to synthetic and experimental data, and suggest limits for the accessible kinetic range. We conclude that equilibrium constants obtained from SV and SE analysis are equivalent, but that SV experiments provide better confidence for the Kd, can better account for the presence of contaminants and provide additional information including rate constants and shape parameters. (Graph Presented)

Original languageEnglish (US)
Pages (from-to)775-782
Number of pages8
JournalMacromolecular Bioscience
Volume10
Issue number7
DOIs
StatePublished - Jul 7 2010

Keywords

  • Analytical ultracentrifugation
  • Equilibrium complexes and rate constants
  • Monomers
  • Polycomb repression complex 1
  • RING1B
  • Reversible associations
  • Sedimentation velocity
  • Structure
  • Ultrascan

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomaterials
  • Polymers and Plastics
  • Materials Chemistry

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