Characterization of rat kidney proximal tubule brush border membrane-associated phosphatidylinositol phosphodiesterase

Dorie W. Schwertz, Jeffrey I. Kreisberg, Manjeri A Venkatachalam

Research output: Contribution to journalArticle

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Abstract

Isolated rat kidney proximal tubule brush border membrane vesicles exhibit an increase in diacylglycerol levels (20- to 30-fold) and a concomitant decrease in phosphatidylinositol when incubated with [3H]arachidonate-labeled lipids, Ca2+, and deoxycholate. Levels of free arachidonate, triglyceride, and noninositol phospholipids are not altered. These results suggest phosphatidylinositol phosphodiesterase activity is associated with rat proximal tubule brush border membrane. Presence of both deoxycholate and certain divalent cations was necessary to demonstrate enzyme activity. Optimum pH ranged from 7.0 to 8.5. Ca2+, Mg2+, and Mn2+ stimulated diglyceride production while Ba2+, Zn2+, Hg2+, and K+ were ineffective. HgCl2 inhibited Ca2+-stimulated phosphatidylinositol phosphodiesterase. Mg2+ and deoxycholate-dependent enzyme activity was shown to be phosphatidylinositol specific. Sodium lauryl sulfate, tetradecyltrimethylammonium bromide, and Triton X-100 did not activate phosphatidylinositol phosphodiesterase in the presence of Ca2+. In combination with deoxycholate, diglyceride formation was not affected by sodium lauryl sulfate, partially inhibited by Triton X-100, and completely abolished by tetradecyltrimethylammonium bromide. Diglyceride kinase activity was not found associated with brush border membrane phosphatidylinositol phosphodiesterase. ATP (1-5 mm) inhibited Ca2+- or Mg2+-stimulated, deoxycholate-dependent phosphatidylinositol hydrolysis by chelating the required divalent cation.

Original languageEnglish (US)
Pages (from-to)555-567
Number of pages13
JournalArchives of Biochemistry and Biophysics
Volume224
Issue number2
DOIs
StatePublished - Jul 15 1983

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Proximal Kidney Tubule
Phosphoric Diester Hydrolases
Brushes
Microvilli
Phosphatidylinositols
Rats
Deoxycholic Acid
Membranes
Diglycerides
Divalent Cations
Octoxynol
Enzyme activity
Sodium Dodecyl Sulfate
Diacylglycerol Kinase
Mercuric Chloride
Enzymes
Chelation
Hydrolysis
Phospholipids
Triglycerides

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Characterization of rat kidney proximal tubule brush border membrane-associated phosphatidylinositol phosphodiesterase. / Schwertz, Dorie W.; Kreisberg, Jeffrey I.; Venkatachalam, Manjeri A.

In: Archives of Biochemistry and Biophysics, Vol. 224, No. 2, 15.07.1983, p. 555-567.

Research output: Contribution to journalArticle

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