Abstract
Pseudomonas chlororaphis phage 201φ{symbol}2-1 is a relative of Pseudomonas aeruginosa myovirus φ{symbol}KZ. Phage 201φ{symbol}2-1 was examined by complete genomic sequencing (316,674 bp), by a comprehensive mass spectrometry survey of its virion proteins and by electron microscopy. Seventy-six proteins, of which at least 69 have homologues in φ{symbol}KZ, were identified by mass spectrometry. Eight proteins, in addition to the major head, tail sheath and tail tube proteins, are abundant in the virion. Electron microscopy of 201φ{symbol}2-1 revealed a multitude of long, fine fibers apparently decorating the tail sheath protein. Among the less abundant virion proteins are three homologues to RNA polymerase β or β′ subunits. Comparison between the genomes of 201φ{symbol}2-1 and φ{symbol}KZ revealed substantial conservation of the genome plan, and a large region with an especially high rate of gene replacement. The φ{symbol}KZ-like phages exhibited a two-fold higher rate of divergence than for T4-like phages or host genomes.
Original language | English (US) |
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Pages (from-to) | 330-338 |
Number of pages | 9 |
Journal | Virology |
Volume | 376 |
Issue number | 2 |
DOIs | |
State | Published - Jul 5 2008 |
Keywords
- Accelerated divergence
- Bacteriophage
- RNA polymerase
- Virion proteins
ASJC Scopus subject areas
- Virology