Characterization of pressure transients generated by nanosecond electrical pulse (nsEP) exposure

Caleb C. Roth, Ronald A. Barnes, Bennett L. Ibey, Hope T. Beier, L. Christopher Mimun, Saher M. Maswadi, Mehdi Shadaram, Randolph D. Glickman

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The mechanism(s) responsible for the breakdown (nanoporation) of cell plasma membranes after nanosecond pulse (nsEP) exposure remains poorly understood. Current theories focus exclusively on the electrical field, citing electrostriction, water dipole alignment and/or electrodeformation as the primary mechanisms for pore formation. However, the delivery of a high-voltage nsEP to cells by tungsten electrodes creates a multitude of biophysical phenomena, including electrohydraulic cavitation, electrochemical interactions, thermoelastic expansion, and others. To date, very limited research has investigated non-electric phenomena occurring during nsEP exposures and their potential effect on cell nanoporation. Of primary interest is the production of acoustic shock waves during nsEP exposure, as it is known that acoustic shock waves can cause membrane poration (sonoporation). Based on these observations, our group characterized the acoustic pressure transients generated by nsEP and determined if such transients played any role in nanoporation. In this paper, we show that nsEP exposures, equivalent to those used in cellular studies, are capable of generating high-frequency (2.5MHz), high-intensity (>13kPa) pressure transients. Using confocal microscopy to measure cell uptake of YO-PRO®-1 (indicator of nanoporation of the plasma membrane) and changing the electrode geometry, we determined that acoustic waves alone are not responsible for poration of the membrane.

Original languageEnglish (US)
Article number15063
JournalScientific Reports
Volume5
DOIs
StatePublished - Oct 9 2015

Fingerprint

Cell Membrane
Pressure
Biophysical Phenomena
Electrodes
Tungsten
Membranes
Acoustics
Confocal Microscopy
Water
Research
YO-PRO 1

ASJC Scopus subject areas

  • General

Cite this

Roth, C. C., Barnes, R. A., Ibey, B. L., Beier, H. T., Christopher Mimun, L., Maswadi, S. M., ... Glickman, R. D. (2015). Characterization of pressure transients generated by nanosecond electrical pulse (nsEP) exposure. Scientific Reports, 5, [15063]. https://doi.org/10.1038/srep15063

Characterization of pressure transients generated by nanosecond electrical pulse (nsEP) exposure. / Roth, Caleb C.; Barnes, Ronald A.; Ibey, Bennett L.; Beier, Hope T.; Christopher Mimun, L.; Maswadi, Saher M.; Shadaram, Mehdi; Glickman, Randolph D.

In: Scientific Reports, Vol. 5, 15063, 09.10.2015.

Research output: Contribution to journalArticle

Roth, CC, Barnes, RA, Ibey, BL, Beier, HT, Christopher Mimun, L, Maswadi, SM, Shadaram, M & Glickman, RD 2015, 'Characterization of pressure transients generated by nanosecond electrical pulse (nsEP) exposure', Scientific Reports, vol. 5, 15063. https://doi.org/10.1038/srep15063
Roth CC, Barnes RA, Ibey BL, Beier HT, Christopher Mimun L, Maswadi SM et al. Characterization of pressure transients generated by nanosecond electrical pulse (nsEP) exposure. Scientific Reports. 2015 Oct 9;5. 15063. https://doi.org/10.1038/srep15063
Roth, Caleb C. ; Barnes, Ronald A. ; Ibey, Bennett L. ; Beier, Hope T. ; Christopher Mimun, L. ; Maswadi, Saher M. ; Shadaram, Mehdi ; Glickman, Randolph D. / Characterization of pressure transients generated by nanosecond electrical pulse (nsEP) exposure. In: Scientific Reports. 2015 ; Vol. 5.
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