TY - JOUR
T1 - Characterization of new simian foamy viruses from African nonhuman primates
AU - Broussard, Suzanne R.
AU - Comuzzie, Anthony G.
AU - Leighton, Karen L.
AU - Leland, M. Michelle
AU - Whitehead, Evelyn M.
AU - Allan, Jonathan S.
N1 - Funding Information:
We thank Dr. Richard Heberling at The Virus Reference Lab for providing the HFV isolate and HFSM cells, Pablo Nieves at the Carri-bean Primate Research Center, Amy Shima and Kim Williams at the San Diego Zoo for providing blood samples from African primates, and Jo Fletcher for review of this manuscript. This study was funded by the National Institute of Health Grant AI-28273.
PY - 1997/10/27
Y1 - 1997/10/27
N2 - Simian foamy viruses (SFV) are exogenous retroviruses present in most if not all nonhuman primate species. Baboons and other African monkey species are known to harbor SFVs, yet there is presently no data in regard to their genetic relationship. Here we studied SFVs from baboons as compared to other SFVs isolated from a Hamlyn's guenon, a patas monkey, and a vervet. By Western blot analysis, the gag precursor proteins (p74/p70) were detected from all SFVs. In addition, the envelope glycoproteins from a vervet isolate (SFV-Agm2) were comparable in size to the env precursor gp130, the exterior glycoprotein (gp70), and the transmembrane protein (gp48) as detected by lentil lectin binding and radioimmunoprecipitation (RIPA). Molecular comparison of PCR amplified products from pol and LTR regions of each SFV demonstrated a close relationship among baboon SFVs white SFVs from patas, Hamlyn's guenon, and vervet clustered together. The baboon viruses only Varied by 4% among each other in the LTR region; however, as much as 26% variation was noted when compared to the other African monkey SFVs. To determine the prevalence rate of SFV-Bab in our baboon colony, we employed both Western blotting and PCR analysis. Antibodies to SFV gag precursor proteins were seen in 7 of 10 infants; however, none were positive by PCR, suggesting that these infants were virus negative and that their antibodies were maternal in origin. Only one juvenile (1/10) and all adults (38/38) were infected with SFV. Taken together these results suggest that SFVs have arisen and diverged along with the evolution of their natural hosts. Furthermore, the high prevalence rates to SFV seen in adult baboons strongly suggest a sexual or oral routes of transmission.
AB - Simian foamy viruses (SFV) are exogenous retroviruses present in most if not all nonhuman primate species. Baboons and other African monkey species are known to harbor SFVs, yet there is presently no data in regard to their genetic relationship. Here we studied SFVs from baboons as compared to other SFVs isolated from a Hamlyn's guenon, a patas monkey, and a vervet. By Western blot analysis, the gag precursor proteins (p74/p70) were detected from all SFVs. In addition, the envelope glycoproteins from a vervet isolate (SFV-Agm2) were comparable in size to the env precursor gp130, the exterior glycoprotein (gp70), and the transmembrane protein (gp48) as detected by lentil lectin binding and radioimmunoprecipitation (RIPA). Molecular comparison of PCR amplified products from pol and LTR regions of each SFV demonstrated a close relationship among baboon SFVs white SFVs from patas, Hamlyn's guenon, and vervet clustered together. The baboon viruses only Varied by 4% among each other in the LTR region; however, as much as 26% variation was noted when compared to the other African monkey SFVs. To determine the prevalence rate of SFV-Bab in our baboon colony, we employed both Western blotting and PCR analysis. Antibodies to SFV gag precursor proteins were seen in 7 of 10 infants; however, none were positive by PCR, suggesting that these infants were virus negative and that their antibodies were maternal in origin. Only one juvenile (1/10) and all adults (38/38) were infected with SFV. Taken together these results suggest that SFVs have arisen and diverged along with the evolution of their natural hosts. Furthermore, the high prevalence rates to SFV seen in adult baboons strongly suggest a sexual or oral routes of transmission.
KW - African green monkey
KW - Baboon
KW - Hamlyn's guenon
KW - Patas
KW - Phylogeny
KW - SFV
KW - Spumavirus
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U2 - 10.1006/viro.1997.8797
DO - 10.1006/viro.1997.8797
M3 - Article
C2 - 9356346
AN - SCOPUS:0030664073
VL - 237
SP - 349
EP - 359
JO - Virology
JF - Virology
SN - 0042-6822
IS - 2
ER -