Characterization of mutant Ha-ras gene expression in transformed murine keratinocyte lines grown under in vitro and in vivo conditions

John P.G. Volpe, Claudio J. Conti, Thomas J. Slaga

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

We investigated the Ha-ras and Ki-ras gene status, tumorigenicity, pathology, line derivation, and intercellular communication of 7,12-dimethylbenz[a]anthracene-initiated papilloma-, carcinoma-, and hyperplastic skin-producing cell lines to further characterize them. Six of nine tumor cell lines grown in vitro expressed both mutant and normal Ha-ras proteins, and three lines expressed only normal Ha-ras. However, when grown subcutaneously in nude mice, seven of the nine lines expressed both mutant and normal Ha-ras, one line expressed normal Ha-ras, and one line did not grow subcutaneously. One papilloma line, P2/15, appeared to have an inducible mutant Ha-ras gene, as it was expressed only in vivo. These findings suggest that mutant Ha-ras genes may be lost in only a minor population of tumor lines during growth in culture. Finally, we found that mutant Ha-ras gene expression was strongly correlated with tumorigenicity in nude mice and that intercellular communication was strongly correlated with the derivation of the lines.

Original languageEnglish (US)
Pages (from-to)202-206
Number of pages5
JournalMolecular Carcinogenesis
Volume17
Issue number4
DOIs
StatePublished - Dec 1 1996
Externally publishedYes

Keywords

  • papilloma
  • ras
  • squamous cell carcinoma
  • tumor cell lines

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research

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