Characterization of multiple promoters directing tissue-specific expression of the human gonadotropin-releasing hormone gene

Ke Wen Dong, Kei Li Yu, Zheng Guang Chen, Ya Di Chen, James L. Roberts

Research output: Contribution to journalArticlepeer-review

35 Scopus citations

Abstract

Two promoters directing tissue-specific expression of GnRH gene in neuronal and reproductive tissues were characterized by functional analyses of GnRH promoter-luciferase (LUC) constructs in transfected placental cells (JEG) and hypothalamic neuronal cells (GT1-7). Results indicate that the downstream promoter directs the expression in a neuronal cell-specific manner, whereas the upstream promoter is fully active in the nonneural placental cell line. Transfection studies carried out in several tumor cell lines derived from human reproductive tissues verified that the upstream GnRH promoter construct was much more active in directing luciferase expression in reproductive tissue. The use of both upstream and downstream promoters in various human tumor cell lines derived from reproductive tissues were demonstrated by RT-PCR. Our studies also demonstrate that the reproductive tissue-specific messenger RNA transcribed from upstream promoter is capable of directing synthesis of preproGnRH protein. Serial deletion studies localized a cell-specific upstream promoter region that directs reproductive tissue expression, DNase I footprint analysis using nuclear extract obtained from the JEG cells indicated DNA/protein interactions in four specific sequence elements of the upstream promoter region. The interaction between nuclear binding proteins present in the JEG cells (but not the GT1-7 cells) and the four specific sequences in the upstream promoter region was confirmed by gel mobility shift analysis.

Original languageEnglish (US)
Pages (from-to)2754-2762
Number of pages9
JournalEndocrinology
Volume138
Issue number7
DOIs
StatePublished - 1997

ASJC Scopus subject areas

  • Endocrinology

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