Characterization of lytic Pseudomonas aeruginosa bacteriophages via biological properties and genomic sequences

Natia Karumidze, Julie A. Thomas, Nino Kvatadze, Marina Goderdzishvili, Kevin W. Hakala, Susan T. Weintraub, Zemphira Alavidze, Stephen C. Hardies

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

Pseudomonas aeruginosa is an important cause of infections, especially in patients with immunodeficiency or diabetes. Antibiotics are effective in preventing morbidity and mortality from Pseudomonas infection, but because of spreading multidrug-resistant bacterial strains, bacteriophages are being explored as an alternative therapy. Two newly purified broad host range Pseudomonas phages, named vB-Pae-Kakheti25 and vB-Pae-TbilisiM32, were characterized as candidates for use in phage therapy. Morphology, host range, growth properties, thermal stability, serology, genomic sequence, and virion composition are reported. When phages are used as bactericides, they are used in mixtures to overcome the development of resistance in the targeted bacterial population. These two phages are representative of diverse siphoviral and podoviral phage families, respectively, and hence have unrelated mechanisms of infection and no cross-antigenicity. Composing bactericidal phage mixtures with members of different phage families may decrease the incidence of developing resistance through a common mechanism.

Original languageEnglish (US)
Pages (from-to)1609-1617
Number of pages9
JournalApplied Microbiology and Biotechnology
Volume94
Issue number6
DOIs
StatePublished - May 8 2012

Keywords

  • Bacteriophage
  • Host range
  • Phage resistance
  • Phage therapy
  • Pseudomonas aeruginosa

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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    Karumidze, N., Thomas, J. A., Kvatadze, N., Goderdzishvili, M., Hakala, K. W., Weintraub, S. T., Alavidze, Z., & Hardies, S. C. (2012). Characterization of lytic Pseudomonas aeruginosa bacteriophages via biological properties and genomic sequences. Applied Microbiology and Biotechnology, 94(6), 1609-1617. https://doi.org/10.1007/s00253-012-4119-8