Separation of platelets from plasma is achieved by adding ADP (final concentration 10-5 M) to platelet rich plasma and allowing aggregates to form. Aggregates are removed quickly by brief, gentle centrifugation, washed 2 to 3 times with 0.9% NaCl (saline), and then incubated for 10 min in the presence of apyrase, albumin and calcium. Platelet aggregates deaggregate completely during this incubation period. The platelet suspension is then subjected to 1100g for 12 min, gently resuspended in a small volume of saline, and finally diluted with an appropriate medium to the desired concentration. The entire separation procedure requires approximately 30 min. Platelets obtained by this procedure are comparable in aggregability to the platelet preparations obtained by gel filtration, have normal intracellular amounts of ATP and ADP, and except for slight dilatation of the surface connected canalicular system, have normal ultrastructural appearance. When suspended in an appropriate medium, these separated platelets take up serotonin 14C and subsequently release it in nearly normal quantities when exposed to thrombin, collagen or ADP.
|Original language||English (US)|
|Number of pages||14|
|Journal||American Journal of Pathology|
|State||Published - 1975|
ASJC Scopus subject areas
- Pathology and Forensic Medicine