Characterization of hemoglobin from the backswimmer Buenoa margaritacea (Hemiptera: Notonectidae) reveals high electrophoretic heterogeneity

Charles R. Vossbrinck, Pawel A. Osmulski, Peter G. Debrunner

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Backswimmers (Notonectidae) are one of the three families of insects which contain species known to make hemoglobin. In backswimmers the hemoglobin allows the insect to maintain a prolonged neutral buoyancy during its dive. Sephadex G-200 column chromatography, SDS-polyacrylamide gel electrophoresis and HPLC demonstrate a molecular weight for the monomeric hemoglobin og Buenoa margaritacea of 15,000-19,000 Da. u.v.-visible spectra at eight hemoglobin derivatives (Fe2+:deoxy, O2, CO, NO; Fe3+:H2O, OH-, CN-, imidazole) are given. Native gel electrophoresis of the cyanomet form shows nine bands, while isoelectric focusing shows 12. Bands isolated from preparative IEF gels form multiple bands when rerun, indicative of a change in aggregation status after isolation. Interactions among various hemoglobin components are seen using 2-dimensional "titration" electrophoresis.

Original languageEnglish (US)
Pages (from-to)421-429
Number of pages9
JournalInsect Biochemistry and Molecular Biology
Volume23
Issue number3
DOIs
StatePublished - Apr 1993

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Keywords

  • Anisops
  • Backswimmer
  • Buenoa margaritacea
  • Bug hemoglobin
  • Hemoglobin
  • Insect
  • Notonectidae
  • Respiration

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Insect Science

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