Characterization of autoantigenic sites on isolated dog heart mitochondria

Robert E. Kelley, Merle S. Olson, R. Neal Pinckard

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

1. 1. Anti-heart mitochondria autoantibodies were developed in serum from dogs following experimental myocardial infarction. 2. 2. Heart mitochondria frozen and thawed repeatedly in a sucrose/Tris-chloride buffer retained both their functional integrity as measured by the respiratory control ratio and their ability to serve as an antigen in a complement fixation test. Mitochondria frozen and thawed in a potassium chloride/Tris-chloride buffer lost both their functional integrity and their autoantigenic activity after one freeze-thaw cycle. 3. 3. Extraction of the heart mitochondria with acetone/water mixtures to remove phospholipids from the membrane led to a complete loss of the ability of the mitochondria to react in the complement fixation test but did not affect the ability of the membranes to bind autoantibody in absorption experiments. 4. 4. Treatment of the mitochondrial membranes with increasing concentrations of trypsin caused a loss of up to approximately 50% of the membrane protein with a gradual decrease in the autoantigenic activity of the membrane without impairment of the ability of the membrane to bind autoantibody. 5. 5. Removal of up to 90% of the sialic acid of the mitochondrial membrane with neuraminidase resulted in a considerable increase in the complement-fixing autoantigenic activity of the membrane without changing the apparent ability of the membrane to bind autoantibody in absorption experiments. 6. 6. Exposure of mitochondrial membranes to autoantibody and complement caused an inhibition of both an inner mitochondrial membrane enzyme, i.e. cytochrome oxidase (48%) and an outer mitochondrial membrane enzyme, i.e. NADH-cytochrome c reductase (rotenone insensitive) (37%).

Original languageEnglish (US)
Pages (from-to)370-385
Number of pages16
JournalBBA - Biomembranes
Volume401
Issue number3
DOIs
StatePublished - Sep 2 1975
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology

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