Characterization of a unique ClpB protein of Mycoplasma pneumoniae and its impact on growth

Thirumalai R Kannan, Oxana Musatovova, Pramod Gowda, Joel B. Baseman

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Mycoplasma pneumoniae accounts for 20 to 30% of all community-acquired pneumonia and has been associated with other airway pathologies, including asthma, and a range of extrapulmonary manifestations. Although the entire genomic sequence of M. pneumoniae has been completed, the functions of many of these genes in mycoplasma physiology are unknown. In this study, we focused on clpB, a well-known heat shock gene in other bacteria, to examine its role in mycoplasma growth. Transcriptional and translational analyses of heat shock in M. pneumoniae indicated that clpB is significantly upregulated, reinforcing its status as a critical responder to heat stress. Interestingly, M. pneumoniae ClpB does not use dual translational start points for ClpB synthesis, like other ClpB-characterized bacteria. Biochemical characterization of purified M. pneumoniae recombinant ClpB revealed casein- and lysine-independent ATPase activity and DnaK-DnaJ-GrpEdependent chaperone activity. An M. pneumoniae mini-Tn4001-integrated, clpB-null mutant was impaired in its ability to replicate under permissive growth conditions, demonstrating the growth-promoting status of ClpB.

Original languageEnglish (US)
Pages (from-to)5082-5092
Number of pages11
JournalInfection and Immunity
Volume76
Issue number11
DOIs
StatePublished - Nov 2008

Fingerprint

Mycoplasma pneumoniae
Growth
Proteins
Mycoplasma
Hot Temperature
Shock
Bacteria
Caseins
Genes
Lysine
Adenosine Triphosphatases
Pneumonia
Asthma
Pathology

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Parasitology
  • Infectious Diseases

Cite this

Characterization of a unique ClpB protein of Mycoplasma pneumoniae and its impact on growth. / Kannan, Thirumalai R; Musatovova, Oxana; Gowda, Pramod; Baseman, Joel B.

In: Infection and Immunity, Vol. 76, No. 11, 11.2008, p. 5082-5092.

Research output: Contribution to journalArticle

Kannan, Thirumalai R ; Musatovova, Oxana ; Gowda, Pramod ; Baseman, Joel B. / Characterization of a unique ClpB protein of Mycoplasma pneumoniae and its impact on growth. In: Infection and Immunity. 2008 ; Vol. 76, No. 11. pp. 5082-5092.
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