Characterization of a unique ADP-ribosyltransferase of Mycoplasma penetrans

Coreen Johnson, Thirumalai R Kannan, Joel B. Baseman

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Mycoplasma penetrans is a urogenital tract pathogen implicated in the deterioration of the immune system in human immunodeficiency virus-infected AIDS patients. Here, we describe a 78-kDa protein from M. penetrans, designated MYPE9110, that exhibits sequence similarity to known ADP-ribosyltransferases (ADPRTs) such as Bordetella pertussis pertussis toxin and Mycoplasma pneumoniae community-acquired respiratory distress syndrome toxin. MYPE9110 possesses key amino acid residues found in all ADPRTs that are essential for ADPRT activity. Several mammalian cell proteins are ADP-ribosylated by MYPE9110, and the full-length recombinant protein exhibits a strong auto-ADP-ribosylating activity. In the absence of target proteins, MYPE9110 demonstrates a NAD-glycohydrolase activity by hydrolyzing NAD. Furthermore, this toxin elicits cytopathology in HeLa cells by inducing cytoplasmic vacuolization in the presence of ammonium chloride. The deletion of the C-terminal region of MYPE9110 significantly diminishes its binding to host cells while still exhibiting an ADPRT activity, suggesting that MYPE9110 is a member of the family of A-B ADPRT toxins.

Original languageEnglish (US)
Pages (from-to)4362-4370
Number of pages9
JournalInfection and Immunity
Volume77
Issue number10
DOIs
StatePublished - Oct 2009

Fingerprint

Mycoplasma penetrans
ADP Ribose Transferases
Adenosine Diphosphate
NAD+ Nucleosidase
Ammonium Chloride
Bordetella pertussis
Mycoplasma pneumoniae
Proteins
Pertussis Toxin
HeLa Cells
Recombinant Proteins
NAD
Immune System
Acquired Immunodeficiency Syndrome
HIV
Amino Acids

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Parasitology
  • Infectious Diseases

Cite this

Characterization of a unique ADP-ribosyltransferase of Mycoplasma penetrans. / Johnson, Coreen; Kannan, Thirumalai R; Baseman, Joel B.

In: Infection and Immunity, Vol. 77, No. 10, 10.2009, p. 4362-4370.

Research output: Contribution to journalArticle

Johnson, Coreen ; Kannan, Thirumalai R ; Baseman, Joel B. / Characterization of a unique ADP-ribosyltransferase of Mycoplasma penetrans. In: Infection and Immunity. 2009 ; Vol. 77, No. 10. pp. 4362-4370.
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