TY - JOUR
T1 - Characterization of a novel zinc finger gene with increased expression in nondividing normal human cells
AU - Ran, Qitao
AU - Wadhwa, Renu
AU - Bischof, Oliver
AU - Venable, Susan
AU - Smith, James R.
AU - Pereira-Smith, Olivia M.
PY - 2001/2/1
Y1 - 2001/2/1
N2 - We report here the cloning and characterization of a novel KRAB zinc finger gene, ZFQR, which has eight tandemly repeated zinc fingers, a complete KRAB box at the N-terminal region, and a unique C-terminal sequence. It is expressed in a variety of human tissues, and mRNA levels are upregulated in nondividing senescent and quiescent human fibroblasts. Overexpression of the protein in quiescent cells stimulated with serum growth factors results in inhibition of entry into the cell cycle. The latter activity is lost when the N-terminal KRAB domain is deleted. The KRAB domain is also required for the transcriptional repression activity of ZFQR and in maintaining association of the protein with the nuclear matrix. The gene has been mapped to human chromosome 19q13.4. The association of ZFQR with the nuclear matrix, transcriptional repression activity, increased expression in senescent and quiescent cells, and the ability to inhibit quiescent cells stimulated with growth factors from entering the cell cycle suggests a role for ZFQR in the maintenance of the nondividing state of normal human cells.
AB - We report here the cloning and characterization of a novel KRAB zinc finger gene, ZFQR, which has eight tandemly repeated zinc fingers, a complete KRAB box at the N-terminal region, and a unique C-terminal sequence. It is expressed in a variety of human tissues, and mRNA levels are upregulated in nondividing senescent and quiescent human fibroblasts. Overexpression of the protein in quiescent cells stimulated with serum growth factors results in inhibition of entry into the cell cycle. The latter activity is lost when the N-terminal KRAB domain is deleted. The KRAB domain is also required for the transcriptional repression activity of ZFQR and in maintaining association of the protein with the nuclear matrix. The gene has been mapped to human chromosome 19q13.4. The association of ZFQR with the nuclear matrix, transcriptional repression activity, increased expression in senescent and quiescent cells, and the ability to inhibit quiescent cells stimulated with growth factors from entering the cell cycle suggests a role for ZFQR in the maintenance of the nondividing state of normal human cells.
KW - Cell senescence
KW - Nuclear matrix
KW - Quiescence
KW - Transcriptional repression
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U2 - 10.1006/excr.2000.5068
DO - 10.1006/excr.2000.5068
M3 - Article
C2 - 11161714
AN - SCOPUS:0035242369
SN - 0014-4827
VL - 263
SP - 156
EP - 162
JO - Experimental Cell Research
JF - Experimental Cell Research
IS - 1
ER -