Characterization of a HTLV-I-infected cell line derived from a patient with adult T-cell leukemia with stable co-expression of CD4 and CD8

Thomas Rowe, Charlene Dezzutti, Patricia C. Guenthner, Lee Lam, Thomas Hodge, Michael D. Lairmore, Renu B. Lal, Thomas M. Folks

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    13 Scopus citations

    Abstract

    A long-term T-cell line, termed SP+, was developed from a human T-cell leukemia virus type I (HTLV-I)-infected patient with adult T-cell leukemia that is dependent on exogenous IL-2 for growth. The SP+ expresses a full complimentation of HTLV-I-specifrc viral proteins, and contains replication competent viral particles. Restriction enzyme digestion followed by Southern blot analysis demonstrated the presence of a single integrated proviral copy and limiting dilution analysis confirmed the clonality of the cell line. Interestingly, phenotypically, the SP+ cell line is CD2+, CD3+ and coexpresses CD4 and CD8, yet lacks TCRαβ and TCRτδ expression. Further ontogenetic characterization of the SP+ cell line demonstrated the lack of thymic T-cell precursor markers, including absence of cell surface expression of CD1, intracellular thymic terminal deoxynucleotidyl transferase (TdT) enzyme, as well as message expression for V(D)J recombinase activating gene-1 (RAG-1). Furthermore, the SP+ cell did express the message for the CD3δ chain. Taken together, these data suggest that the SP+ cell line resulted from HTLV-I infection of a mature CD4+/CDB+ lymphocyte. This cell line can be potentially useful as a model, both for regulation of cellular functions by HTLV-I and for immunologic functions of mature dual CD4/CD8 positive T-cells.

    Original languageEnglish (US)
    Pages (from-to)621-628
    Number of pages8
    JournalLeukemia Research
    Volume19
    Issue number9
    DOIs
    Publication statusPublished - Sep 1995

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    Keywords

    • ATL
    • cell line
    • dual CD CD8 positive

    ASJC Scopus subject areas

    • Hematology
    • Oncology
    • Cancer Research

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