Characterization of a composite gradient gel for the electrophoretic separation of lipoproteins

David L. Rainwater, Perry H. Moore, Wendy R. Shelledy, Thomas D. Dyer, Susan H. Slifer

Research output: Contribution to journalArticlepeer-review

74 Scopus citations

Abstract

We describe a protocol for making a new type of gradient gel, the Composite gradient gel, that was designed to resolve plasma lipoproteins using nondenaturing gradient gel electrophoresis. The new gel format allows analysis both of high density lipoproteins (HDLs) and low density lipoproteins (LDLs) on the same gel. The gel gave highly repeatable (r2=0.999) size estimates. We compared lipoprotein phenotypes determined from the new gradient gel with those obtained using specialized HDL and LDL gradient gels. The comparisons indicated that the Composite gel gave lipoprotein particle size estimates for HDLs and LDLs that were virtually identical to those obtained, respectively, from the specialized HDL and LDL gradient gels. We measured median diameters, which reflect the distributions of absorbance, for LDLs and for HDLs and found that the Composite gel gave lipoprotein size distributions that were virtually identical to those measured using the specialized LDL and HDL gels. Finally, comparison of fractional absorbance for six lipoprotein size intervals obtained from the Composite and specialized gels revealed a close correlation (r2=0.828). Thus, it appears that both LDL and HDL size phenotypes may be evaluated simultaneously using a single gradient gel format.

Original languageEnglish (US)
Pages (from-to)1261-1266
Number of pages6
JournalJournal of lipid research
Volume38
Issue number6
StatePublished - Jun 1997
Externally publishedYes

Keywords

  • Baboon
  • Gradient gel electrophoresis
  • High density lipoprotein
  • Human
  • Low density lipoprotein

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Cell Biology

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