A carotenoidless, blue mutant of Rhodospirillum rubrum strain S1 was isolated and characterized with respect to its nutritional requirements, growth kinetics and absorption spectra. The mutant contained bacteriochlorophyll a and the carotenoid phytoene, required reduced sulfur compounds for growth under photosynthetic conditions, and produced H2S when sulfur was supplied as cystine. The specific growth rate, bacteriochlorophyll a, and protein content of the mutant were comparable to its wild type parent under most conditions; however, when the mutant was transferred from photosynthetic to chemo-organotrophic conditions in the light, the mutant rapidly lost viability.
ASJC Scopus subject areas
- Molecular Biology