Characterization of a 54-nucleotide gap region in the 28S rRNA gene of Schistosoma mansoni

Harry van Keulen, Prema M. Mertz, Philip T. LoVerde, Hong Shi, David M. Rekosh

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

We have analyzed 572 bp in the 28S rDNA of the human blood fluke Schistosoma mansoni which correspond to expansion segment 5 of domain IV as defined by Clark et al. [12] for the Xenopus laevis 28S rRNA. S1 nuclease mapping and primer extension analysis comparing this region with the mature 28S rRNA indicate that there are 54 nucleotides present in the 28S rDNA which are absent from the mature rRNA. This defines a gap that creates two 28S rRNA subunits (28Sα and 28Sβ). Comparison of the S. mansoni sequence with rDNAs of other organisms which contain gaps in their 28S rRNA shows that the overall features are conserved except that the S. mansoni gap is less A+T-rich. The conserved features include: (1) the location of the gap within the 28S rRNA; (2) the predicted secondary structure of the gap, containing a stem-loop with a UAAU sequence within the loop; and (3) a conserved CGAAAGGG on the 3′ side of the gap.

Original languageEnglish (US)
Pages (from-to)205-214
Number of pages10
JournalMolecular and Biochemical Parasitology
Volume45
Issue number2
DOIs
StatePublished - Apr 1991

Keywords

  • 28S rRNA
  • Gap sequence
  • Schistosoma mansoni
  • rDNA

ASJC Scopus subject areas

  • Parasitology
  • Molecular Biology

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