Characterization of γδT cells in naïve and HIV-infected chimpanzees and their responses to T-cell activators in vitro

Vida L. Hodara, Laura M. Parodi, Deborah Chavez, Lisa M. Smith, Robert Lanford, Luis D. Giavedoni

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Background: γδT cells are effector cells that eliminate cancer and virus-infected cells. Chimpanzees are an endangered species that can naturally and experimentally be infected with SIV and HIV, respectively, but no information about the functionality of γδT cells during chronic lentiviral infection is currently available. Methods: Healthy and HIV-infected chimpanzee γδT cells were characterized by flow cytometry. γδT subsets were studied after stimulation with T-cell activators, and the release of cytokines was analyzed by Luminex assay. Results: γδT-cell subsets, Vδ1 and Vδ2Vγ9, showed different patterns in the expression of CD4, CD195, CD159a, and CD159c. Stimulation of γδT cells resulted in increased levels of CD4 and HLA-DR, which is more pronounced in Vδ1 T cells. Distinct cytokine patterns were found between healthy and HIV-infected chimpanzees. Conclusions: Analyses of major chimpanzee γδT subsets show similarities to human γδT cells and suggest different functionality and roles in their immune response against HIV infection.

Original languageEnglish (US)
Pages (from-to)258-271
Number of pages14
JournalJournal of medical primatology
Volume43
Issue number4
DOIs
StatePublished - Aug 2014
Externally publishedYes

Keywords

  • Chimpanzee
  • Gamma/delta
  • HIV/AIDS

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

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