Characterization of β-adrenoceptors in the Syrian hamster Harderian gland: Sexual differences and effects of either castration or superior cervical ganglionectomy

A. Pangerl, B. Pangerl, G. R. Buzzell, D. J. Jones, Russel J Reiter

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14 Citations (Scopus)

Abstract

Scatchard analysis of saturation isotherms of [125I]-iodopindolol was used to characterize β-adrenoceptor density (B(max)) and affinity constant (K(d)) in female Harderian glands. Single-point experiments were also completed in intact females, intact males, and castrated or superior cervical ganglionectomized males. Scatchard analysis described a single population of binding sites with a B(max) of 292.2 ± 45.1 fmol/mg protein (X̄ ± SEM = 6) in females and 18.2 ± 3.0 fmol/mg protein (n = 6, P < .001) in males. The affinity also varied significantly (P < .05) with a K(d) of 1.08 ± 0.18 versus 0.26 + 0.05 nM (n = 6) in the Harderian gland of females and males, respectively. Single-point [125I]-IPN (400 pM) binding values in females were 67.3 ± 4.0, in intact males were 12.8 ± 3.2, and in castrated males were 31.2 ± 4.2 fmol/mg protein (n = 7-9). Superior cervical ganglionectomy induced no significant changes in receptor binding. The results indicate pronounced sexual differences in the density and affinity of β-adrenoceptors in the hamster Harderian gland, which may be sex hormone dependent.

Original languageEnglish (US)
Pages (from-to)456-460
Number of pages5
JournalJournal of Neuroscience Research
Volume22
Issue number4
StatePublished - 1989

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Harderian Gland
Ganglionectomy
Castration
Mesocricetus
Adrenergic Receptors
Proteins
Gonadal Steroid Hormones
Cricetinae
Binding Sites
Population

ASJC Scopus subject areas

  • Neuroscience(all)

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Characterization of β-adrenoceptors in the Syrian hamster Harderian gland : Sexual differences and effects of either castration or superior cervical ganglionectomy. / Pangerl, A.; Pangerl, B.; Buzzell, G. R.; Jones, D. J.; Reiter, Russel J.

In: Journal of Neuroscience Research, Vol. 22, No. 4, 1989, p. 456-460.

Research output: Contribution to journalArticle

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