Characterization, mapping, and expression of the human ceruloplasmin gene

F. Yang, S. L. Naylor, J. B. Lum, S. Cutshaw, J. L. McCombs, K. H. Naberhaus, J. R. McGill, G. S. Adrian, C. M. Moore, D. R. Barnett

Research output: Contribution to journalArticle

104 Citations (Scopus)

Abstract

Ceruloplasmin (CP) is a copper-binding protein in vertebrate plasma. It is the product of an intragenic triplication and is composed of three homologous domains. Oligonucleotide probes constructed according to published amino acid sequences were used to identify cDNA clones encoding human CP. Two clones, CP-1 and CP-2, differed from each other by the presence or absence, respectively, of a deduced sequence of four amino acids. The two clones provided 81% of the sequence encoding CP. Comparison of the nucleotides of the three domains of the CP coding sequence revealed internal domain homology with identity of sequences ranging from 50.1% to 56%. The nucleotide sequence of CP-2 cDNA was compared to that of a homologous human protein, clotting factor VIII, and was found to be 48% identical overall. The CP gene was mapped to human chromosome 3 by somatic-cell-hybrid analysis and to 3q25 in situ hybridization; however, sites of hybridization to DNA on other chromosomal sites suggested additional CP-like DNA sequences in the human genome. A DNA polymorphism was detected with CP cDNA after endonuclease digestion of human DNA by Pst I. CP mRNA was detected in human liver, macrophages, and lymphocytes by in situ histohybridization.

Original languageEnglish (US)
Pages (from-to)3257-3261
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume83
Issue number10
StatePublished - 1986
Externally publishedYes

Fingerprint

Ceruloplasmin
Genes
Complementary DNA
Clone Cells
Amino Acid Sequence
DNA
Chromosomes, Human, Pair 3
Blood Coagulation Factors
Oligonucleotide Probes
Hybrid Cells
Endonucleases
Factor VIII
Human Chromosomes
Human Genome
In Situ Hybridization
Vertebrates
Digestion
Nucleotides
Macrophages
Lymphocytes

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Yang, F., Naylor, S. L., Lum, J. B., Cutshaw, S., McCombs, J. L., Naberhaus, K. H., ... Barnett, D. R. (1986). Characterization, mapping, and expression of the human ceruloplasmin gene. Proceedings of the National Academy of Sciences of the United States of America, 83(10), 3257-3261.

Characterization, mapping, and expression of the human ceruloplasmin gene. / Yang, F.; Naylor, S. L.; Lum, J. B.; Cutshaw, S.; McCombs, J. L.; Naberhaus, K. H.; McGill, J. R.; Adrian, G. S.; Moore, C. M.; Barnett, D. R.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 83, No. 10, 1986, p. 3257-3261.

Research output: Contribution to journalArticle

Yang, F, Naylor, SL, Lum, JB, Cutshaw, S, McCombs, JL, Naberhaus, KH, McGill, JR, Adrian, GS, Moore, CM & Barnett, DR 1986, 'Characterization, mapping, and expression of the human ceruloplasmin gene', Proceedings of the National Academy of Sciences of the United States of America, vol. 83, no. 10, pp. 3257-3261.
Yang, F. ; Naylor, S. L. ; Lum, J. B. ; Cutshaw, S. ; McCombs, J. L. ; Naberhaus, K. H. ; McGill, J. R. ; Adrian, G. S. ; Moore, C. M. ; Barnett, D. R. / Characterization, mapping, and expression of the human ceruloplasmin gene. In: Proceedings of the National Academy of Sciences of the United States of America. 1986 ; Vol. 83, No. 10. pp. 3257-3261.
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