Kharakteristika éndogennogo proteoliza v preparatakh membran éritrotsitov cheloveka.

Translated title of the contribution: Characteristics of endogenous proteolysis in preparations of human erythrocyte membranes

P. S. Gromov, S. F. Zakharov, M. Gaczynska, M. Soszyñski, G. Bartosz

Research output: Contribution to journalArticlepeer-review

Abstract

Endoproteolytic activity in human erythrocyte membrane preparations has been examined at 37 degrees C by one- and two-dimensional electrophoresis. Two-dimensional mapping has shown that the presence of leukocyte enzymes in erythrocytes prepared in a regular manner (centrifugation) cannot be excluded. Sedimentation in the 1.5% dextran 500,000 with the following erythrocyte purification on HBS-cellulose has made it possible to prepare erythrocyte membranes characterized by low level endoproteolytic activity without leukocyte enzymes. The marker peptide has been found. It is likely to be a specific product of the enzyme activity of membrane localization.

Translated title of the contributionCharacteristics of endogenous proteolysis in preparations of human erythrocyte membranes
Original languageRussian
Pages (from-to)292-297
Number of pages6
JournalByulleten Eksperimentalnoi Biologii i Meditsiny
Volume106
Issue number9
StatePublished - Sep 1 1988
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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