Endoproteolytic activity in human erythrocyte membrane preparations has been examined at 37 degrees C by one- and two-dimensional electrophoresis. Two-dimensional mapping has shown that the presence of leukocyte enzymes in erythrocytes prepared in a regular manner (centrifugation) cannot be excluded. Sedimentation in the 1.5% dextran 500,000 with the following erythrocyte purification on HBS-cellulose has made it possible to prepare erythrocyte membranes characterized by low level endoproteolytic activity without leukocyte enzymes. The marker peptide has been found. It is likely to be a specific product of the enzyme activity of membrane localization.
|Translated title of the contribution||Characteristics of endogenous proteolysis in preparations of human erythrocyte membranes|
|Number of pages||6|
|Journal||Byulleten Eksperimentalnoi Biologii i Meditsiny|
|State||Published - Sep 1 1988|
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)