Abstract
Endoproteolytic activity in human erythrocyte membrane preparations has been examined at 37 degrees C by one- and two-dimensional electrophoresis. Two-dimensional mapping has shown that the presence of leukocyte enzymes in erythrocytes prepared in a regular manner (centrifugation) cannot be excluded. Sedimentation in the 1.5% dextran 500,000 with the following erythrocyte purification on HBS-cellulose has made it possible to prepare erythrocyte membranes characterized by low level endoproteolytic activity without leukocyte enzymes. The marker peptide has been found. It is likely to be a specific product of the enzyme activity of membrane localization.
| Translated title of the contribution | Characteristics of endogenous proteolysis in preparations of human erythrocyte membranes |
|---|---|
| Original language | Russian |
| Pages (from-to) | 292-297 |
| Number of pages | 6 |
| Journal | Byulleten Eksperimentalnoi Biologii i Meditsiny |
| Volume | 106 |
| Issue number | 9 |
| State | Published - Sep 1988 |
| Externally published | Yes |
ASJC Scopus subject areas
- General Biochemistry, Genetics and Molecular Biology