Characteristics of alkaline phosphatase from two continuous lines of human choriocarcinoma cells

K. V. Speeg; J. C. Azizkhan; K. Stromberg

doi:10.1016/0014-4827(77)90166-5

Characteristics of alkaline phosphatase from two continuous lines of human choriocarcinoma cells

K. V. Speeg, J. C. Azizkhan, K. Stromberg

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Abstract

Several characteristics of human choriocarcinoma alkaline phosphatase have been studied. BeWo and Jar choriocarcinoma lines each contain heat-stable alkaline phosphatase activity with characteristics similar to the D-variant term placental isoenzyme. The activity is also similar to the Nagao enzyme found in many malignancies. Each choriocarcinoma also has heat-labile alkaline phosphatase activity with characteristics similar to the enzyme found in normal first-trimester placenta. Neither BeWo alkaline phosphatase could be stimulated with cortisol. Both BeWo alkaline phosphatase activities could be stimulated with methotrexate and actinomycin D.

Original language	English (US)
Pages (from-to)	199-205
Number of pages	7
Journal	Experimental Cell Research
Volume	105
Issue number	1
DOIs	https://doi.org/10.1016/0014-4827(77)90166-5
State	Published - Mar 1 1977
Externally published	Yes

ASJC Scopus subject areas

Cell Biology

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author = "Speeg, {K. V.} and Azizkhan, {J. C.} and K. Stromberg",

year = "1977",

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AU - Azizkhan, J. C.

AU - Stromberg, K.

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N2 - Several characteristics of human choriocarcinoma alkaline phosphatase have been studied. BeWo and Jar choriocarcinoma lines each contain heat-stable alkaline phosphatase activity with characteristics similar to the D-variant term placental isoenzyme. The activity is also similar to the Nagao enzyme found in many malignancies. Each choriocarcinoma also has heat-labile alkaline phosphatase activity with characteristics similar to the enzyme found in normal first-trimester placenta. Neither BeWo alkaline phosphatase could be stimulated with cortisol. Both BeWo alkaline phosphatase activities could be stimulated with methotrexate and actinomycin D.

AB - Several characteristics of human choriocarcinoma alkaline phosphatase have been studied. BeWo and Jar choriocarcinoma lines each contain heat-stable alkaline phosphatase activity with characteristics similar to the D-variant term placental isoenzyme. The activity is also similar to the Nagao enzyme found in many malignancies. Each choriocarcinoma also has heat-labile alkaline phosphatase activity with characteristics similar to the enzyme found in normal first-trimester placenta. Neither BeWo alkaline phosphatase could be stimulated with cortisol. Both BeWo alkaline phosphatase activities could be stimulated with methotrexate and actinomycin D.

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Characteristics of alkaline phosphatase from two continuous lines of human choriocarcinoma cells

Abstract

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