Characteristics of a human monoclonal anti-Sm autoantibody expressing an interspecies idiotype

M. Takei, H. Dang, R. J. Wang, N. Talal

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

A human-human hybridoma secreting an anti-Sm mAb designated 4B4 was established by fusion of GM4672 (a lymphoblastoid B cell line) with PWM-activated mononuclear cells from a patient with active SLE. Competitive Ag inhibition assays showed that 4B4 was specific for Sm and did not bind with native or denatured DNA or RNA. Western blot analysis with 4B4 showed that this mAb binds to the B/B' ribonucleoprotein of the Sm/ribonucleoprotein complex. By competitive inhibition assay, 4B4 was demonstrated to partially share idiotypic expression with a mouse anti-Sm mAb designated Y2. This was demonstrated by the ability of each mAb (Y2 or 4B4) to inhibit a homologous anti-idiotypic antibody (either anti-Y2 or anti-4B4) better than the nonhomologous anti-idiotypic antibody. These results confirm previous findings that idiotypes related to Sm-binding are highly conserved in nature. Furthermore, this report is the first idiotypic analysis of human anti-Sm mAb.

Original languageEnglish (US)
Pages (from-to)3108-3113
Number of pages6
JournalJournal of Immunology
Volume140
Issue number9
StatePublished - 1988

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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