Abstract
The development and survival of the helminth parasite, Schistosoma mansoni, is dependent on its ability to interpret signals from its environment. Currently, little is known about signal transduction in schistosomes. Rho is a member of a super-family of small GTP-binding proteins. Rho is involved in a number of cell signalling pathways with effects on actin cytoskeleton organisation, gene transcription, cell cycle progression, and membrane trafficking. We have cloned an S. mansoni protein (Rho1) that has 71-75% identity and ∼85% similarity with human Rho A, B, and C proteins. We have optimised expression of recombinant S. mansoni Rho1 protein in Escherichia coli by co-expression with rare tRNAs. Western blot analysis results showed expression of Rho1 protein in adult worm stages especially female worms. In vitro prenylation of recombinant S. mansoni Rho1 determined that, similar to Rho from other organisms, Rho1 is geranynlgeranylated but not farnesylated. A search of the gene database indicates that Rho GTPases exist as a small family in S. mansoni including orthologues of Rho, Cdc42, and Rac. These data suggest that S. mansoni Rho 1 plays a role in signalling in adult worms, especially females.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 721-731 |
| Number of pages | 11 |
| Journal | International Journal for Parasitology |
| Volume | 33 |
| Issue number | 7 |
| DOIs | |
| State | Published - Jul 2003 |
| Externally published | Yes |
Fingerprint
Keywords
- Cdc42
- Prenylation
- Rac
- Rho
- Schistosoma mansoni
- Small GTPase
ASJC Scopus subject areas
- Parasitology
- Infectious Diseases
Cite this
Characterisation of a Rho homologue of Schistosoma mansoni. / Vermeire, Jon J.; Osman, Ahmed; Loverde, Philip T; Williams, David L.
In: International Journal for Parasitology, Vol. 33, No. 7, 07.2003, p. 721-731.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Characterisation of a Rho homologue of Schistosoma mansoni
AU - Vermeire, Jon J.
AU - Osman, Ahmed
AU - Loverde, Philip T
AU - Williams, David L.
PY - 2003/7
Y1 - 2003/7
N2 - The development and survival of the helminth parasite, Schistosoma mansoni, is dependent on its ability to interpret signals from its environment. Currently, little is known about signal transduction in schistosomes. Rho is a member of a super-family of small GTP-binding proteins. Rho is involved in a number of cell signalling pathways with effects on actin cytoskeleton organisation, gene transcription, cell cycle progression, and membrane trafficking. We have cloned an S. mansoni protein (Rho1) that has 71-75% identity and ∼85% similarity with human Rho A, B, and C proteins. We have optimised expression of recombinant S. mansoni Rho1 protein in Escherichia coli by co-expression with rare tRNAs. Western blot analysis results showed expression of Rho1 protein in adult worm stages especially female worms. In vitro prenylation of recombinant S. mansoni Rho1 determined that, similar to Rho from other organisms, Rho1 is geranynlgeranylated but not farnesylated. A search of the gene database indicates that Rho GTPases exist as a small family in S. mansoni including orthologues of Rho, Cdc42, and Rac. These data suggest that S. mansoni Rho 1 plays a role in signalling in adult worms, especially females.
AB - The development and survival of the helminth parasite, Schistosoma mansoni, is dependent on its ability to interpret signals from its environment. Currently, little is known about signal transduction in schistosomes. Rho is a member of a super-family of small GTP-binding proteins. Rho is involved in a number of cell signalling pathways with effects on actin cytoskeleton organisation, gene transcription, cell cycle progression, and membrane trafficking. We have cloned an S. mansoni protein (Rho1) that has 71-75% identity and ∼85% similarity with human Rho A, B, and C proteins. We have optimised expression of recombinant S. mansoni Rho1 protein in Escherichia coli by co-expression with rare tRNAs. Western blot analysis results showed expression of Rho1 protein in adult worm stages especially female worms. In vitro prenylation of recombinant S. mansoni Rho1 determined that, similar to Rho from other organisms, Rho1 is geranynlgeranylated but not farnesylated. A search of the gene database indicates that Rho GTPases exist as a small family in S. mansoni including orthologues of Rho, Cdc42, and Rac. These data suggest that S. mansoni Rho 1 plays a role in signalling in adult worms, especially females.
KW - Cdc42
KW - Prenylation
KW - Rac
KW - Rho
KW - Schistosoma mansoni
KW - Small GTPase
UR - http://www.scopus.com/inward/record.url?scp=0038177917&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0038177917&partnerID=8YFLogxK
U2 - 10.1016/S0020-7519(03)00046-8
DO - 10.1016/S0020-7519(03)00046-8
M3 - Article
C2 - 12814652
AN - SCOPUS:0038177917
VL - 33
SP - 721
EP - 731
JO - International Journal for Parasitology
JF - International Journal for Parasitology
SN - 0020-7519
IS - 7
ER -