Characterisation of a Rho homologue of Schistosoma mansoni

Jon J. Vermeire, Ahmed Osman, Philip T Loverde, David L. Williams

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The development and survival of the helminth parasite, Schistosoma mansoni, is dependent on its ability to interpret signals from its environment. Currently, little is known about signal transduction in schistosomes. Rho is a member of a super-family of small GTP-binding proteins. Rho is involved in a number of cell signalling pathways with effects on actin cytoskeleton organisation, gene transcription, cell cycle progression, and membrane trafficking. We have cloned an S. mansoni protein (Rho1) that has 71-75% identity and ∼85% similarity with human Rho A, B, and C proteins. We have optimised expression of recombinant S. mansoni Rho1 protein in Escherichia coli by co-expression with rare tRNAs. Western blot analysis results showed expression of Rho1 protein in adult worm stages especially female worms. In vitro prenylation of recombinant S. mansoni Rho1 determined that, similar to Rho from other organisms, Rho1 is geranynlgeranylated but not farnesylated. A search of the gene database indicates that Rho GTPases exist as a small family in S. mansoni including orthologues of Rho, Cdc42, and Rac. These data suggest that S. mansoni Rho 1 plays a role in signalling in adult worms, especially females.

Original languageEnglish (US)
Pages (from-to)721-731
Number of pages11
JournalInternational Journal for Parasitology
Volume33
Issue number7
DOIs
StatePublished - Jul 2003
Externally publishedYes

Fingerprint

Schistosoma mansoni
Prenylation
rho GTP-Binding Proteins
Helminths
Escherichia coli Proteins
Transfer RNA
Protein C
Actin Cytoskeleton
GTP-Binding Proteins
Genes
Signal Transduction
Cell Cycle
Parasites
Proteins
Cell Count
Western Blotting
Cell Membrane
Databases

Keywords

  • Cdc42
  • Prenylation
  • Rac
  • Rho
  • Schistosoma mansoni
  • Small GTPase

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

Characterisation of a Rho homologue of Schistosoma mansoni. / Vermeire, Jon J.; Osman, Ahmed; Loverde, Philip T; Williams, David L.

In: International Journal for Parasitology, Vol. 33, No. 7, 07.2003, p. 721-731.

Research output: Contribution to journalArticle

Vermeire, Jon J. ; Osman, Ahmed ; Loverde, Philip T ; Williams, David L. / Characterisation of a Rho homologue of Schistosoma mansoni. In: International Journal for Parasitology. 2003 ; Vol. 33, No. 7. pp. 721-731.
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