TY - JOUR
T1 - Changes Observed in the Growth Fraction, Labeling Index, Duration of S Phase, and Total Cell Cycle Times of hl-60 Cells as They Undergo Differentiation in Response to Retinoic Acid
AU - Gezer, Sefer
AU - Yasin, Zahida
AU - Imren, Suzan
AU - Freeman, James
AU - Black, Ann
AU - Raza, Azra
N1 - Copyright:
Copyright 2016 Elsevier B.V., All rights reserved.
PY - 1988/11
Y1 - 1988/11
N2 - To assess the changes in the proliferative characteristics that occur during maturation, HL-60 cells were induced to differentiate along the granulocytic pathway by retinoic add. Differentiation was documented by morphology, functional markers, and cytochemical staining. Durations of S phase, total cell cycle time, and the percentage of S-phase cells were determined simultaneously at each time point. In addition, the expression of two cell cycle related proteins with molecular weights of 110,000 (p110 measured by monoclonal antibody 5C2) and 145,000 (measured by monoclonal antibody pl45) were measured to estimate the number of cycling cells or the “growth fraction.” Our data demonstrate that as HL-60 cells undergo maturation in response to retinoic acid, a large proportion of cells exit from the cycle, the majority lose their proliferative potential, and the total cell cycle time becomes markedly longer. The slowing of the cell cycle seems to be the result of a prolongation in both S phase and the G1 phase of the cycle. We conclude that more mature myeloid cells cycle more slowly than immature cells. The clinical implications of these findings in myeloid leukemias are discussed.
AB - To assess the changes in the proliferative characteristics that occur during maturation, HL-60 cells were induced to differentiate along the granulocytic pathway by retinoic add. Differentiation was documented by morphology, functional markers, and cytochemical staining. Durations of S phase, total cell cycle time, and the percentage of S-phase cells were determined simultaneously at each time point. In addition, the expression of two cell cycle related proteins with molecular weights of 110,000 (p110 measured by monoclonal antibody 5C2) and 145,000 (measured by monoclonal antibody pl45) were measured to estimate the number of cycling cells or the “growth fraction.” Our data demonstrate that as HL-60 cells undergo maturation in response to retinoic acid, a large proportion of cells exit from the cycle, the majority lose their proliferative potential, and the total cell cycle time becomes markedly longer. The slowing of the cell cycle seems to be the result of a prolongation in both S phase and the G1 phase of the cycle. We conclude that more mature myeloid cells cycle more slowly than immature cells. The clinical implications of these findings in myeloid leukemias are discussed.
UR - http://www.scopus.com/inward/record.url?scp=0023736303&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0023736303&partnerID=8YFLogxK
M3 - Article
C2 - 2844395
AN - SCOPUS:0023736303
VL - 48
SP - 5989
EP - 5994
JO - Cancer Research
JF - Cancer Research
SN - 0008-5472
IS - 21
ER -