Changes in the proteolytic activity of human erythrocyte membrane during red cell aging

Maria Gaczyńska

doi:10.1016/0005-2736(89)90025-4

Changes in the proteolytic activity of human erythrocyte membrane during red cell aging

Maria Gaczyńska

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

The action of endogenous membrane proteinases in membranes isolated from human red cells of various ages was assayed by three groups of methods: (1) determination of the amount of protein fragments released to the acid-soluble fraction; (2) monitoring of changes in ESR spectra of maleimide spin-labeled erythrocyte membranes; (3) electrophoretic methods; a two-dimensional analysis and analysis of the activity inside SDS-PAGE gels. For all the methods the effects of proteinase action were highest in ghosts isolated from the erythrocytes of middle age.

Original language	English (US)
Pages (from-to)	173-177
Number of pages	5
Journal	BBA - Biomembranes
Volume	981
Issue number	2
DOIs	https://doi.org/10.1016/0005-2736(89)90025-4
State	Published - Jun 6 1989
Externally published	Yes

Keywords

Aging
Erythrocyte membrane
Proteolysis

ASJC Scopus subject areas

Biophysics
Biochemistry
Cell Biology

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10.1016/0005-2736(89)90025-4

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title = "Changes in the proteolytic activity of human erythrocyte membrane during red cell aging",

abstract = "The action of endogenous membrane proteinases in membranes isolated from human red cells of various ages was assayed by three groups of methods: (1) determination of the amount of protein fragments released to the acid-soluble fraction; (2) monitoring of changes in ESR spectra of maleimide spin-labeled erythrocyte membranes; (3) electrophoretic methods; a two-dimensional analysis and analysis of the activity inside SDS-PAGE gels. For all the methods the effects of proteinase action were highest in ghosts isolated from the erythrocytes of middle age.",

keywords = "Aging, Erythrocyte membrane, Proteolysis",

author = "Maria Gaczy{\'n}ska",

note = "Funding Information: This work was performed within the framework of Project RP-II-11.4.8 of the Polish Ministry of Education.",

year = "1989",

month = jun,

day = "6",

doi = "10.1016/0005-2736(89)90025-4",

language = "English (US)",

volume = "981",

pages = "173--177",

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T1 - Changes in the proteolytic activity of human erythrocyte membrane during red cell aging

AU - Gaczyńska, Maria

N1 - Funding Information: This work was performed within the framework of Project RP-II-11.4.8 of the Polish Ministry of Education.

PY - 1989/6/6

Y1 - 1989/6/6

N2 - The action of endogenous membrane proteinases in membranes isolated from human red cells of various ages was assayed by three groups of methods: (1) determination of the amount of protein fragments released to the acid-soluble fraction; (2) monitoring of changes in ESR spectra of maleimide spin-labeled erythrocyte membranes; (3) electrophoretic methods; a two-dimensional analysis and analysis of the activity inside SDS-PAGE gels. For all the methods the effects of proteinase action were highest in ghosts isolated from the erythrocytes of middle age.

AB - The action of endogenous membrane proteinases in membranes isolated from human red cells of various ages was assayed by three groups of methods: (1) determination of the amount of protein fragments released to the acid-soluble fraction; (2) monitoring of changes in ESR spectra of maleimide spin-labeled erythrocyte membranes; (3) electrophoretic methods; a two-dimensional analysis and analysis of the activity inside SDS-PAGE gels. For all the methods the effects of proteinase action were highest in ghosts isolated from the erythrocytes of middle age.

KW - Aging

KW - Erythrocyte membrane

KW - Proteolysis

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AN - SCOPUS:0024337770

SN - 0005-2736

VL - 981

SP - 173

EP - 177

JO - BBA - Biomembranes

JF - BBA - Biomembranes

IS - 2

ER -

Changes in the proteolytic activity of human erythrocyte membrane during red cell aging

Abstract

Keywords

ASJC Scopus subject areas

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