The action of endogenous membrane proteinases in membranes isolated from human red cells of various ages was assayed by three groups of methods: (1) determination of the amount of protein fragments released to the acid-soluble fraction; (2) monitoring of changes in ESR spectra of maleimide spin-labeled erythrocyte membranes; (3) electrophoretic methods; a two-dimensional analysis and analysis of the activity inside SDS-PAGE gels. For all the methods the effects of proteinase action were highest in ghosts isolated from the erythrocytes of middle age.
|Original language||English (US)|
|Number of pages||5|
|Journal||BBA - Biomembranes|
|State||Published - Jun 6 1989|
- Erythrocyte membrane
ASJC Scopus subject areas
- Cell Biology