Changes in the proteolytic activity of human erythrocyte membrane during red cell aging

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8 Scopus citations

Abstract

The action of endogenous membrane proteinases in membranes isolated from human red cells of various ages was assayed by three groups of methods: (1) determination of the amount of protein fragments released to the acid-soluble fraction; (2) monitoring of changes in ESR spectra of maleimide spin-labeled erythrocyte membranes; (3) electrophoretic methods; a two-dimensional analysis and analysis of the activity inside SDS-PAGE gels. For all the methods the effects of proteinase action were highest in ghosts isolated from the erythrocytes of middle age.

Original languageEnglish (US)
Pages (from-to)173-177
Number of pages5
JournalBBA - Biomembranes
Volume981
Issue number2
DOIs
StatePublished - Jun 6 1989
Externally publishedYes

Keywords

  • Aging
  • Erythrocyte membrane
  • Proteolysis

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology

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