Changes in protein expression during multistage mouse skin carcinogenesis

Joyce E. Rundhaug, Irma Gimenez-Conti, Mariana C. Stern, Irina V. Budunova, Kaoru Kiguchi, David K. Bol, Lezlee G. Coghlan, Claudio J. Conti, John DiGiovanni, Susan M. Fischer, Lloyd D. Winberg, Thomas J Slaga

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

To directly compare the expression patterns of different proteins known to be altered during mouse skin carcinogenesis, serial sections of normal and hyperplastic skin and tumors from various stages of 7,12- dimethylbenz[a]anthracene-initiated, 12-O-tetradecanoylphorbol-13-acetate- promoted female SENCAR mice were examined by immunohistochemistry. In untreated, normal mouse skin, keratin 1 (K1) and transforming growth factor- β1 (TGFβ1) were strongly expressed in the suprabasal layers, whereas integrin α6β4 was expressed only in basal cells and only moderate staining for transforming growth factor-α (TGFα) was seen. In hyperplastic skin, TGFα expression became stronger, whereas expression of another epidermal growth factor (EGF) receptor ligand, heparin-binding EGF-like growth factor (HB-EGF), was strongly induced in all epidermal layers from no expression in normal skin. Likewise, the gap-junctional protein connexin 26 (Cx26) became highly expressed in the differentiated granular layers of hyperplastic skin relative to undetectable expression in normal skin. Expression of cyclin D1 in the proliferative cell compartment was seen in all benign and malignant tumors but not in hyperplastic skin. Beginning with very early papillomas (after 10 wk of promotion), expression of (α6β4 in suprabasal cells and small, focal staining for keratin 13 (K13) were seen in some tumors. Later (after 20-30 wk), focal areas of 7-glutamyl transpeptidase (GGT) activity appeared in a few papillomas, whereas TGFβ1 expression began to decrease. Cx26 and TGFα staining became patchier in some late-stage papillomas (30-40 wk), whereas suprabasal α6β4, K13, and GGT expression progressively increased and K1 expression decreased. Finally, in squamous cell carcinomas (SCCs), there was an almost complete loss of K1 and a further decline in TGFα, HB-EGF, TGFβ1, and Cx26 expression. On the other hand, almost all SCCs showed suprabasal staining for α6β4 and widespread cyclin D1 and K13 expression, whereas only about half showed positive focal staining for GGT activity.

Original languageEnglish (US)
Pages (from-to)125-136
Number of pages12
JournalMolecular Carcinogenesis
Volume20
Issue number1
DOIs
StatePublished - Sep 1997
Externally publishedYes

Fingerprint

Transforming Growth Factors
Carcinogenesis
Skin
Keratin-13
Keratin-1
Staining and Labeling
Papilloma
Proteins
Cyclin D1
Squamous Cell Carcinoma
Inbred SENCAR Mouse
Neoplasms
gamma-Glutamyltransferase
Tetradecanoylphorbol Acetate
Epidermal Growth Factor Receptor
Epidermal Growth Factor
Integrins
Heparin
Intercellular Signaling Peptides and Proteins
Immunohistochemistry

Keywords

  • Connexin 26
  • Cyclin D1
  • Heparin-binding epidermal growth factor-like growth factor
  • Integrin α6β4
  • Transforming growth factor

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Biology

Cite this

Changes in protein expression during multistage mouse skin carcinogenesis. / Rundhaug, Joyce E.; Gimenez-Conti, Irma; Stern, Mariana C.; Budunova, Irina V.; Kiguchi, Kaoru; Bol, David K.; Coghlan, Lezlee G.; Conti, Claudio J.; DiGiovanni, John; Fischer, Susan M.; Winberg, Lloyd D.; Slaga, Thomas J.

In: Molecular Carcinogenesis, Vol. 20, No. 1, 09.1997, p. 125-136.

Research output: Contribution to journalArticle

Rundhaug, JE, Gimenez-Conti, I, Stern, MC, Budunova, IV, Kiguchi, K, Bol, DK, Coghlan, LG, Conti, CJ, DiGiovanni, J, Fischer, SM, Winberg, LD & Slaga, TJ 1997, 'Changes in protein expression during multistage mouse skin carcinogenesis', Molecular Carcinogenesis, vol. 20, no. 1, pp. 125-136. https://doi.org/10.1002/(SICI)1098-2744(199709)20:1<125::AID-MC14>3.0.CO;2-3
Rundhaug, Joyce E. ; Gimenez-Conti, Irma ; Stern, Mariana C. ; Budunova, Irina V. ; Kiguchi, Kaoru ; Bol, David K. ; Coghlan, Lezlee G. ; Conti, Claudio J. ; DiGiovanni, John ; Fischer, Susan M. ; Winberg, Lloyd D. ; Slaga, Thomas J. / Changes in protein expression during multistage mouse skin carcinogenesis. In: Molecular Carcinogenesis. 1997 ; Vol. 20, No. 1. pp. 125-136.
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