Changes in methylation pattern of NMDA receptor NR2B gene in cortical neurons after chronic ethanol treatment in mice

C. R. Marutha Ravindran; Maharaj K. Ticku

doi:10.1016/j.molbrainres.2003.10.025

Changes in methylation pattern of NMDA receptor NR2B gene in cortical neurons after chronic ethanol treatment in mice

C. R. Marutha Ravindran, Maharaj K. Ticku

Department of Pharmacology

Research output: Contribution to journal › Article › peer-review

63 Scopus citations

Abstract

Chronic ethanol treatment produced an increase in the rate of NR2B gene expression in adult cortex and cultured fetal cortical neurons. To determine the possible molecular mechanism involved in the upregulation of NR2B gene expression, we studied methylation in CpG island in mouse fetal cortical neurons. Our results demonstrate that chronic ethanol administration causes demethylation of cytosine residues in CpG island in cortical neurons which may be responsible for the changes in the expression of NR2B gene.

Original language	English (US)
Pages (from-to)	19-27
Number of pages	9
Journal	Molecular Brain Research
Volume	121
Issue number	1-2
DOIs	https://doi.org/10.1016/j.molbrainres.2003.10.025
State	Published - Feb 5 2004

Keywords

Chronic ethanol treatment
CpG
Demethylation
NR2B gene

ASJC Scopus subject areas

Molecular Biology
Cellular and Molecular Neuroscience

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10.1016/j.molbrainres.2003.10.025

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title = "Changes in methylation pattern of NMDA receptor NR2B gene in cortical neurons after chronic ethanol treatment in mice",

abstract = "Chronic ethanol treatment produced an increase in the rate of NR2B gene expression in adult cortex and cultured fetal cortical neurons. To determine the possible molecular mechanism involved in the upregulation of NR2B gene expression, we studied methylation in CpG island in mouse fetal cortical neurons. Our results demonstrate that chronic ethanol administration causes demethylation of cytosine residues in CpG island in cortical neurons which may be responsible for the changes in the expression of NR2B gene.",

keywords = "Chronic ethanol treatment, CpG, Demethylation, NR2B gene",

author = "{Marutha Ravindran}, {C. R.} and Ticku, {Maharaj K.}",

note = "Funding Information: We thank Drs. A.K. Mehta, S. Kadappakkam and H. Kalluri for excellent help. This work was supported by funds from National Institute of Alcohol Abuse and Alcoholism Grant #AA12297. ",

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AU - Marutha Ravindran, C. R.

AU - Ticku, Maharaj K.

N1 - Funding Information: We thank Drs. A.K. Mehta, S. Kadappakkam and H. Kalluri for excellent help. This work was supported by funds from National Institute of Alcohol Abuse and Alcoholism Grant #AA12297.

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Y1 - 2004/2/5

N2 - Chronic ethanol treatment produced an increase in the rate of NR2B gene expression in adult cortex and cultured fetal cortical neurons. To determine the possible molecular mechanism involved in the upregulation of NR2B gene expression, we studied methylation in CpG island in mouse fetal cortical neurons. Our results demonstrate that chronic ethanol administration causes demethylation of cytosine residues in CpG island in cortical neurons which may be responsible for the changes in the expression of NR2B gene.

AB - Chronic ethanol treatment produced an increase in the rate of NR2B gene expression in adult cortex and cultured fetal cortical neurons. To determine the possible molecular mechanism involved in the upregulation of NR2B gene expression, we studied methylation in CpG island in mouse fetal cortical neurons. Our results demonstrate that chronic ethanol administration causes demethylation of cytosine residues in CpG island in cortical neurons which may be responsible for the changes in the expression of NR2B gene.

KW - Chronic ethanol treatment

KW - CpG

KW - Demethylation

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Changes in methylation pattern of NMDA receptor NR2B gene in cortical neurons after chronic ethanol treatment in mice

Abstract

Keywords

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