Changes in membrane potential of intact adipocytes measured with fluorescent dyes

Frederick Bailey; Nathalie Hill; Tadeusz Malinski; Frederick Kiechle

doi:10.1016/0022-0728(89)87233-X

Changes in membrane potential of intact adipocytes measured with fluorescent dyes

Frederick Bailey
, Nathalie Hill
, Tadeusz Malinski
, Frederick Kiechle

Research output: Contribution to journal › Article › peer-review

Abstract

The change in transmembrane potential of rat adipocytes was measured using the fluorescent probes 3,3′-diethylthiadicarbocyanine iodide, [di-SC₂(5)], and 3,3′-dipropylthiadicarbocyanine iodide, [di-SC₃(5)]. The fluorimetric technique was calibrated by altering the potassium ion concentration while keeping the sum of potassium and sodium ions at a constant concentration of 153 mM. A 3% change in relative fluorescence was equivalent to a membrane potential change of 1 mV. This method was used to measure changes in transmembrane potential by insulin and two insulin-mimetic agents: wheat germ agglutinin and ethanolamine.

Original language	English (US)
Pages (from-to)	333-342
Number of pages	10
Journal	Journal of Electroanalytical Chemistry
Volume	275
Issue number	3
DOIs	https://doi.org/10.1016/0022-0728(89)87233-X
State	Published - Jun 1989
Externally published	Yes

ASJC Scopus subject areas

Analytical Chemistry
General Chemical Engineering
Electrochemistry

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10.1016/0022-0728(89)87233-X

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@article{46ba975d5eda41be8e2843c0035edade,

title = "Changes in membrane potential of intact adipocytes measured with fluorescent dyes",

abstract = "The change in transmembrane potential of rat adipocytes was measured using the fluorescent probes 3,3′-diethylthiadicarbocyanine iodide, [di-SC2(5)], and 3,3′-dipropylthiadicarbocyanine iodide, [di-SC3(5)]. The fluorimetric technique was calibrated by altering the potassium ion concentration while keeping the sum of potassium and sodium ions at a constant concentration of 153 mM. A 3\% change in relative fluorescence was equivalent to a membrane potential change of 1 mV. This method was used to measure changes in transmembrane potential by insulin and two insulin-mimetic agents: wheat germ agglutinin and ethanolamine.",

author = "Frederick Bailey and Nathalie Hill and Tadeusz Malinski and Frederick Kiechle",

note = "Funding Information: a grant from William Beaumont Iiospital",

year = "1989",

month = jun,

doi = "10.1016/0022-0728(89)87233-X",

language = "English (US)",

volume = "275",

pages = "333--342",

journal = "Journal of Electroanalytical Chemistry",

issn = "0022-0728",

publisher = "Elsevier BV",

number = "3",

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TY - JOUR

T1 - Changes in membrane potential of intact adipocytes measured with fluorescent dyes

AU - Bailey, Frederick

AU - Hill, Nathalie

AU - Malinski, Tadeusz

AU - Kiechle, Frederick

N1 - Funding Information: a grant from William Beaumont Iiospital

PY - 1989/6

Y1 - 1989/6

N2 - The change in transmembrane potential of rat adipocytes was measured using the fluorescent probes 3,3′-diethylthiadicarbocyanine iodide, [di-SC2(5)], and 3,3′-dipropylthiadicarbocyanine iodide, [di-SC3(5)]. The fluorimetric technique was calibrated by altering the potassium ion concentration while keeping the sum of potassium and sodium ions at a constant concentration of 153 mM. A 3% change in relative fluorescence was equivalent to a membrane potential change of 1 mV. This method was used to measure changes in transmembrane potential by insulin and two insulin-mimetic agents: wheat germ agglutinin and ethanolamine.

AB - The change in transmembrane potential of rat adipocytes was measured using the fluorescent probes 3,3′-diethylthiadicarbocyanine iodide, [di-SC2(5)], and 3,3′-dipropylthiadicarbocyanine iodide, [di-SC3(5)]. The fluorimetric technique was calibrated by altering the potassium ion concentration while keeping the sum of potassium and sodium ions at a constant concentration of 153 mM. A 3% change in relative fluorescence was equivalent to a membrane potential change of 1 mV. This method was used to measure changes in transmembrane potential by insulin and two insulin-mimetic agents: wheat germ agglutinin and ethanolamine.

UR - https://www.scopus.com/pages/publications/45149143314

UR - https://www.scopus.com/pages/publications/45149143314#tab=citedBy

U2 - 10.1016/0022-0728(89)87233-X

DO - 10.1016/0022-0728(89)87233-X

M3 - Article

AN - SCOPUS:45149143314

SN - 0022-0728

VL - 275

SP - 333

EP - 342

JO - Journal of Electroanalytical Chemistry

JF - Journal of Electroanalytical Chemistry

IS - 3

ER -

Changes in membrane potential of intact adipocytes measured with fluorescent dyes

Abstract

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