Changes in membrane potential of intact adipocytes measured with fluorescent dyes

Frederick Bailey, Nathalie Hill, Tadeusz Malinski, Frederick Kiechle

Research output: Contribution to journalArticlepeer-review

Abstract

The change in transmembrane potential of rat adipocytes was measured using the fluorescent probes 3,3′-diethylthiadicarbocyanine iodide, [di-SC2(5)], and 3,3′-dipropylthiadicarbocyanine iodide, [di-SC3(5)]. The fluorimetric technique was calibrated by altering the potassium ion concentration while keeping the sum of potassium and sodium ions at a constant concentration of 153 mM. A 3% change in relative fluorescence was equivalent to a membrane potential change of 1 mV. This method was used to measure changes in transmembrane potential by insulin and two insulin-mimetic agents: wheat germ agglutinin and ethanolamine.

Original languageEnglish (US)
Pages (from-to)333-342
Number of pages10
JournalJournal of Electroanalytical Chemistry
Volume275
Issue number3
DOIs
StatePublished - Jun 1989
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry
  • Chemical Engineering(all)
  • Electrochemistry

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