Changes in keratin expression during 7,12-dimethylbenz[a]anthracene-induced hamster cheek pouch carcinogenesis

Irma B. Gimenez-Conti, Dong M. Shin, Albert B. Bianchi, Thomas J Slaga, Waun Ki Hong, Claudio J. Conti, Thomas J. Slaga

Research output: Contribution to journalArticle

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Abstract

This study was undertaken to explore the expression of keratins in the hamster cheek pouch carcinogenesis model, using monospecific keratin antibodies and a technique that allows immunoblotting analysis of tissues embedded in paraffin. Changes in keratin expression were correlated with histopathological changes and with the expression of the enzyme γ-glutamyl transpeptidase. The right cheek pouch of 20 male golden Syrian hamsters was treated with 0.5% 7,12-dimethylbenz[a]anthracene for 16 weeks. As previously described by other laboratories, this treatment resulted in hyperplastic and dysplastic lesions and benign and malignant tumors. The keratins assayed in this study were K14 (M, 55,000), K1 (M, 67,000), and K13 (M, 47,000). The normal hamster cheek pouch epithelium expressed K14 in the basal layer and K13 in the suprabasal and differentiated layers, whereas K1 was not detected by either immunohistochemistry or immunoblotting. Concomitant with 7,12-dimethylbenz[a]anthracene-induced hyperplasia, there were some topographical alterations in the distribution of K14. In this case, K14 was no longer restricted to the basal layer but was also expressed in differentiated cells. The same pattern was also observed in dysplastic lesions and in squamous cell carcinoma. Furthermore, expression of the K13 differentiation-associated keratin was preserved in this hyperplastic epithelium during all the stages of carcinogenesis, including either anaplastic or differentiated areas. In contrast, after 2 weeks of 7,12-dimethylbenz[a]anthracene treatment, K1 expression started as a weak and patchy pattern in suprabasal cells, becoming stronger and more homogeneous at 8 and 16 weeks of treatment. However, K1 was almost absent in squamous cell carcinoma, where only small very well differentiated areas were stained. We also observed γ-glutamyl transpeptidase-positive foci in earlier stages of carcinogenesis, concomitant with the expression of the K1 keratin. However, it was not possible to find a perfect topographical correspondence between the two events. Alterations in the pattern of keratin expression appear to be a common feature during the development of squamous cell carcinoma in different systems and could be an excellent tool to study carcinogenesis and chemoprevention.

Original languageEnglish (US)
Pages (from-to)4441-4445
Number of pages5
JournalCancer Research
Volume50
Issue number14
StatePublished - Jul 15 1990
Externally publishedYes

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Cheek
Keratins
Cricetinae
Carcinogenesis
Squamous Cell Carcinoma
gamma-Glutamyltransferase
Immunoblotting
Epithelium
Mesocricetus
Chemoprevention
anthracene
Paraffin
Hyperplasia
Therapeutics
Immunohistochemistry
Antibodies
Enzymes
Neoplasms

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Gimenez-Conti, I. B., Shin, D. M., Bianchi, A. B., Slaga, T. J., Hong, W. K., Conti, C. J., & Slaga, T. J. (1990). Changes in keratin expression during 7,12-dimethylbenz[a]anthracene-induced hamster cheek pouch carcinogenesis. Cancer Research, 50(14), 4441-4445.

Changes in keratin expression during 7,12-dimethylbenz[a]anthracene-induced hamster cheek pouch carcinogenesis. / Gimenez-Conti, Irma B.; Shin, Dong M.; Bianchi, Albert B.; Slaga, Thomas J; Hong, Waun Ki; Conti, Claudio J.; Slaga, Thomas J.

In: Cancer Research, Vol. 50, No. 14, 15.07.1990, p. 4441-4445.

Research output: Contribution to journalArticle

Gimenez-Conti, IB, Shin, DM, Bianchi, AB, Slaga, TJ, Hong, WK, Conti, CJ & Slaga, TJ 1990, 'Changes in keratin expression during 7,12-dimethylbenz[a]anthracene-induced hamster cheek pouch carcinogenesis', Cancer Research, vol. 50, no. 14, pp. 4441-4445.
Gimenez-Conti IB, Shin DM, Bianchi AB, Slaga TJ, Hong WK, Conti CJ et al. Changes in keratin expression during 7,12-dimethylbenz[a]anthracene-induced hamster cheek pouch carcinogenesis. Cancer Research. 1990 Jul 15;50(14):4441-4445.
Gimenez-Conti, Irma B. ; Shin, Dong M. ; Bianchi, Albert B. ; Slaga, Thomas J ; Hong, Waun Ki ; Conti, Claudio J. ; Slaga, Thomas J. / Changes in keratin expression during 7,12-dimethylbenz[a]anthracene-induced hamster cheek pouch carcinogenesis. In: Cancer Research. 1990 ; Vol. 50, No. 14. pp. 4441-4445.
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