Changes in diadenosine tetraphosphate levels in Physarum polycephalum with different oxygen concentrations

P. N. Garrison, S. A. Mathis, L. D. Barnes

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9 Scopus citations

Abstract

Cellular levels of diadenosine tetraphosphate (Ap4A) were measured, by a specific high-pressure liquid chromatography method, in microplasmodia of Physarum polycephalum subjected to different degrees of hypoxia, hyperoxia, and treatment with H2O2. Ap4A levels increased three- to sevenfold under anaerobic conditions, and the microplasmodia remained viable after such treatment. Elevated levels of Ap4A returned to the basal level within 5 to 10 min upon reoxygenation of the microplasmodia. The increases in Ap4A levels were larger in stationary-phase or starved microplasmodia than in fed, log-phase microplasmodia. The maximal increase measured in log-phase microplasmodia was twofold. No significant changes in Ap4A levels occurred in microplasmodia subjected to mild hypoxia, hyperoxia, or treatment with 1 mM H2O2. These results indicate that in P. polycephalum, Ap4A may function in the metabolic response to anaerobic conditions rather than in the response to oxidative stress.

Original languageEnglish (US)
Pages (from-to)1506-1512
Number of pages7
JournalJournal of bacteriology
Volume171
Issue number3
DOIs
StatePublished - 1989

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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