The cell-free protein synthetic activity of the postmitochondrial supernatant isolated from whole brain of 6 to 32 mth old male Fischer F344 rats was compared. Protein synthesis decreased 56% from 6 to 32 mth of age. The decrease in cell-free protein synthesis was not due to an age-related increase in RNase activity. Although monomeric ribosomes (ribosomes stripped of mRNA) isolated from the brains of older rats were less active in polyuridylic acid directed polyphenylalanine synthesis, the fidelity of polyuridylic acid translation by monomeric ribosomes did not decrease with increasing age.
ASJC Scopus subject areas
- Geriatrics and Gerontology