Cerium dioxide nanoparticles synthesized synthesized via precipitation at constant pH: Synthesis, physical-chemical and antioxidant properties

Yuliia Shlapa, Serhii Solopan, Veronika Sarnatskaya, Katarina Siposova, Ivana Garcarova, Katerina Veltruska, Illia Timashkov, Oleksandra Lykhova, Denis Kolesnik, Andrey Musatov, Vladimir Nikolaev, Anatolii Belous

Research output: Contribution to journalArticlepeer-review


Cerium oxide nanoparticles (CeO2 NPs) are well known for their application in various fields of industry, as well as in biology and medicine. Knowledge of synthesis schemes, physicochemical and morphological features of nanoscale CeO2 is important for assessing their antioxidant behavior and understanding the mechanism of oxidative stress and its consequences. The choice of the method of synthesis should be based on the possibility to choose the conditions and parameters for obtaining CeO2 with controlled dimensions and a ratio of Се3+/Се4+ on their surface. In this study, CeO2 NPs are synthesized by precipitation in mixed water-alcohol solutions at constant pH = 9. The properties of obtained NPs are studied using various methods of physical-chemical characterization such as X-ray diffraction, X-ray photoelectron spectroscopy, transmission electron microscopy, and dynamic light scattering. The size of CeO2 NPs varied from 14 to 4.2 nm with increasing alcohol concentration, while the effect of constant pH during synthesis on the morphology of the particles was insignificant. The synthesized nanoparticles form highly stable aqueous suspensions since their zeta-potential is higher than + 40 mV. It is found that the ability of CeO2 NPs to self-stabilize is associated with the presence of hydrated Ce4+ ions on their surface. In vitro biological studies have shown that, regardless of particle size, CeO2 NPs have antioxidant potential, but smaller NPs with a higher percentage of Ce3+ on the surface had a more effective antioxidant effect. In addition, the size-depended activity of CeO2 NPs to inhibit the amyloid formation of insulin is demonstrated.

Original languageEnglish (US)
Article number112960
JournalColloids and Surfaces B: Biointerfaces
StatePublished - Dec 2022
Externally publishedYes


  • Anti-amyloid activity
  • Antioxidant properties
  • Cerium dioxide nanoparticles
  • Characterization
  • Constant pH
  • Synthesis

ASJC Scopus subject areas

  • Biotechnology
  • Surfaces and Interfaces
  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry


Dive into the research topics of 'Cerium dioxide nanoparticles synthesized synthesized via precipitation at constant pH: Synthesis, physical-chemical and antioxidant properties'. Together they form a unique fingerprint.

Cite this