Abstract
The centromere plays an essential role in accurate chromosome segregation, and the chromosomal location of the centromere is determined by the presence of a histone H3 variant, centromere protein A (CENP-A), in centromeric nucleosomes. However, the precise mechanisms of deposition, maintenance, and inheritance of CENP-A at centromeres are unclear. We have reported that CENP-A deposition requires ubiquitylation of CENP-A lysine 124 mediated by the E3 ligase activity of Cullin 4A (CUL4A)—RING-box protein 1 (RBX1)—COP9 signalsome complex subunit 8 (COPS8). We have proposed a model of inheritance for CENP-A ubiquitylation, through dimerization between rounds of cell divisions, that maintains the position of centromeres.
Original language | English (US) |
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Article number | 402 |
Journal | Molecules |
Volume | 24 |
Issue number | 3 |
DOIs | |
State | Published - Jan 22 2019 |
Keywords
- CENP-A
- CUL4A-RBX1- COPS8 E3 ligase
- Centromere
- Centromere identity
- Dimerization
- Epigenetics
- Kinetochore
- Lysine 124 (K124), neocentromere
- Ubiquitylation
ASJC Scopus subject areas
- Drug Discovery
- Analytical Chemistry
- Chemistry (miscellaneous)
- Molecular Medicine
- Physical and Theoretical Chemistry
- Pharmaceutical Science
- Organic Chemistry