CENP-A ubiquitylation contributes to maintaining the chromosomal location of the centromere

Yohei Niikura, Risa Kitagawa, Katsumi Kitagawa

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

The centromere plays an essential role in accurate chromosome segregation, and the chromosomal location of the centromere is determined by the presence of a histone H3 variant, centromere protein A (CENP-A), in centromeric nucleosomes. However, the precise mechanisms of deposition, maintenance, and inheritance of CENP-A at centromeres are unclear. We have reported that CENP-A deposition requires ubiquitylation of CENP-A lysine 124 mediated by the E3 ligase activity of Cullin 4A (CUL4A)—RING-box protein 1 (RBX1)—COP9 signalsome complex subunit 8 (COPS8). We have proposed a model of inheritance for CENP-A ubiquitylation, through dimerization between rounds of cell divisions, that maintains the position of centromeres.

Original languageEnglish (US)
Article number402
JournalMolecules
Volume24
Issue number3
DOIs
StatePublished - Jan 22 2019

Keywords

  • CENP-A
  • CUL4A-RBX1- COPS8 E3 ligase
  • Centromere
  • Centromere identity
  • Dimerization
  • Epigenetics
  • Kinetochore
  • Lysine 124 (K124), neocentromere
  • Ubiquitylation

ASJC Scopus subject areas

  • Analytical Chemistry
  • Chemistry (miscellaneous)
  • Molecular Medicine
  • Pharmaceutical Science
  • Drug Discovery
  • Physical and Theoretical Chemistry
  • Organic Chemistry

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