CENP-A ubiquitylation contributes to maintaining the chromosomal location of the centromere

Yohei Niikura; Risa Kitagawa; Katsumi Kitagawa

doi:10.3390/molecules24030402

CENP-A ubiquitylation contributes to maintaining the chromosomal location of the centromere

Yohei Niikura, Risa Kitagawa, Katsumi Kitagawa

Department of Molecular Medicine

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

The centromere plays an essential role in accurate chromosome segregation, and the chromosomal location of the centromere is determined by the presence of a histone H3 variant, centromere protein A (CENP-A), in centromeric nucleosomes. However, the precise mechanisms of deposition, maintenance, and inheritance of CENP-A at centromeres are unclear. We have reported that CENP-A deposition requires ubiquitylation of CENP-A lysine 124 mediated by the E3 ligase activity of Cullin 4A (CUL4A)—RING-box protein 1 (RBX1)—COP9 signalsome complex subunit 8 (COPS8). We have proposed a model of inheritance for CENP-A ubiquitylation, through dimerization between rounds of cell divisions, that maintains the position of centromeres.

Original language	English (US)
Article number	402
Journal	Molecules
Volume	24
Issue number	3
DOIs	https://doi.org/10.3390/molecules24030402
State	Published - Jan 22 2019

Keywords

CENP-A
CUL4A-RBX1- COPS8 E3 ligase
Centromere
Centromere identity
Dimerization
Epigenetics
Kinetochore
Lysine 124 (K124), neocentromere
Ubiquitylation

ASJC Scopus subject areas

Analytical Chemistry
Chemistry (miscellaneous)
Molecular Medicine
Pharmaceutical Science
Drug Discovery
Physical and Theoretical Chemistry
Organic Chemistry

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title = "CENP-A ubiquitylation contributes to maintaining the chromosomal location of the centromere",

abstract = "The centromere plays an essential role in accurate chromosome segregation, and the chromosomal location of the centromere is determined by the presence of a histone H3 variant, centromere protein A (CENP-A), in centromeric nucleosomes. However, the precise mechanisms of deposition, maintenance, and inheritance of CENP-A at centromeres are unclear. We have reported that CENP-A deposition requires ubiquitylation of CENP-A lysine 124 mediated by the E3 ligase activity of Cullin 4A (CUL4A)—RING-box protein 1 (RBX1)—COP9 signalsome complex subunit 8 (COPS8). We have proposed a model of inheritance for CENP-A ubiquitylation, through dimerization between rounds of cell divisions, that maintains the position of centromeres.",

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author = "Yohei Niikura and Risa Kitagawa and Katsumi Kitagawa",

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AU - Niikura, Yohei

AU - Kitagawa, Risa

AU - Kitagawa, Katsumi

PY - 2019/1/22

Y1 - 2019/1/22

N2 - The centromere plays an essential role in accurate chromosome segregation, and the chromosomal location of the centromere is determined by the presence of a histone H3 variant, centromere protein A (CENP-A), in centromeric nucleosomes. However, the precise mechanisms of deposition, maintenance, and inheritance of CENP-A at centromeres are unclear. We have reported that CENP-A deposition requires ubiquitylation of CENP-A lysine 124 mediated by the E3 ligase activity of Cullin 4A (CUL4A)—RING-box protein 1 (RBX1)—COP9 signalsome complex subunit 8 (COPS8). We have proposed a model of inheritance for CENP-A ubiquitylation, through dimerization between rounds of cell divisions, that maintains the position of centromeres.

AB - The centromere plays an essential role in accurate chromosome segregation, and the chromosomal location of the centromere is determined by the presence of a histone H3 variant, centromere protein A (CENP-A), in centromeric nucleosomes. However, the precise mechanisms of deposition, maintenance, and inheritance of CENP-A at centromeres are unclear. We have reported that CENP-A deposition requires ubiquitylation of CENP-A lysine 124 mediated by the E3 ligase activity of Cullin 4A (CUL4A)—RING-box protein 1 (RBX1)—COP9 signalsome complex subunit 8 (COPS8). We have proposed a model of inheritance for CENP-A ubiquitylation, through dimerization between rounds of cell divisions, that maintains the position of centromeres.

KW - CENP-A

KW - CUL4A-RBX1- COPS8 E3 ligase

KW - Centromere

KW - Centromere identity

KW - Dimerization

KW - Epigenetics

KW - Kinetochore

KW - Lysine 124 (K124), neocentromere

KW - Ubiquitylation

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DO - 10.3390/molecules24030402

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JO - Molecules

JF - Molecules

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ER -

CENP-A ubiquitylation contributes to maintaining the chromosomal location of the centromere

Abstract

Keywords

ASJC Scopus subject areas

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