Cellular refractive index comparison of various prostate cancer and noncancerous cell lines via photonic-crystal biosensor

Frank Deluna, Melissa Cadena, Bingzhi Wang, Lu Zhe Sun, Jing Yong Ye

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The current clinical standard for mass screening of prostate cancer are prostate-specific antigen (PSA) biomarker assays. Unfortunately, the low specificity of PSA's bioassays to prostate cancer leads to high false-positive rates, as such there is an urgent need for the development of a more specific detection system independent of PSA levels. In our previous research, we have successfully demonstrated, with the use of our Photonic-Crystal based biosensor in a Total-Internal-Reflection (PC-TIR) configuration, detection of prostate cancer (PC-3) cells against benign prostate hyperplasia (BPH-1) cells. The PC-TIR biosensor achieved detection of individual prostate cancer cells utilizing cellular refractive index (RI) as the only contrast parameter. To further study this methodology in vitro, we report a comprehensive study of the cellular RI's of various prostate cancer and noncancerous cell lines (i.e. RWPE-1, BPH-1, PC-3, DU-145, and LNCaP) via reflectance spectroscopy and single-cell RI imaging utilizing the PC-TIR biosensor. Our study shows promising clinical potential in utilizing the PC-TIR biosensor system for the detection of prostate cancer against noncancerous prostate epithelial cells.

Original languageEnglish (US)
Title of host publicationImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVII
EditorsJames F. Leary, Attila Tarnok, Daniel L. Farkas
PublisherSPIE
ISBN (Electronic)9781510624047
DOIs
StatePublished - Jan 1 2019
EventImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVII 2019 - San Francisco, United States
Duration: Feb 4 2019Feb 6 2019

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume10881
ISSN (Print)1605-7422

Conference

ConferenceImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVII 2019
CountryUnited States
CitySan Francisco
Period2/4/192/6/19

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Keywords

  • Cancer Diagnostics
  • Cellular Refractive Index
  • Label-Free Imaging
  • Optical Biosensors
  • Photonic-Crystal Biosensor
  • Prostate Cancer

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

Cite this

Deluna, F., Cadena, M., Wang, B., Sun, L. Z., & Ye, J. Y. (2019). Cellular refractive index comparison of various prostate cancer and noncancerous cell lines via photonic-crystal biosensor. In J. F. Leary, A. Tarnok, & D. L. Farkas (Eds.), Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVII [108810K] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 10881). SPIE. https://doi.org/10.1117/12.2507505