Cellular localization of the folate receptor: Potential role in drug toxicity and folate homeostasis

Steven D Weitman, Arthur G. Weinberg, Leslie R. Coney, Vincent R. Zurawski, Debra S. Jennings, Barton A. Kamen

Research output: Contribution to journalArticle

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Abstract

In the past year, gp38, a glycosyl-phosphatidylinositol linked membrane protein that is overexpressed in some malignant tissues, has been shown to be the folate receptor. Using immunohistochemical techniques with the monoclonal antibody MOv19 against gp38, we evaluated the cellular localization of folate receptors in normal human tissues, which are potential target sites for drugs that utilize this uptake mechanism. The choroid plexus was intensely positive with staining limited to the epithelium, which in some foci had a distinct bilaminar pattern limited to the luminal and basal surfaces. The epithelium of the fallopian tube, uterus, and epididymis was highly immunoreactive. The acinar cells of the breast, submandibular salivary, and bronchial glands also showed intense staining as did the trophoblastic cells of the placenta. In the kidney reactivity was localized to the proximal tubules. Lung alveolar lining including type I and II pneumocytes stained intensely. Limited but focal reactivity was noted in the vas deferens, ovary, thyroid, and pancreas. This study in conjunction with previous work showing marked overexpression of folate receptor in some malignant cells suggests that the folate receptor may be an important target for diagnostic or therapeutic exploitation and indicates sites of potential drug toxicity.

Original languageEnglish (US)
Pages (from-to)6708-6711
Number of pages4
JournalCancer Research
Volume52
Issue number23
StatePublished - Dec 1 1992
Externally publishedYes

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Drug-Related Side Effects and Adverse Reactions
Folic Acid
Homeostasis
Alveolar Epithelial Cells
Epithelium
Staining and Labeling
Glycosylphosphatidylinositols
Choroid Plexus
Vas Deferens
Fallopian Tubes
Acinar Cells
Epididymis
Salivary Glands
Placenta
Uterus
Pancreas
Ovary
Thyroid Gland
Membrane Proteins
Breast

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Weitman, S. D., Weinberg, A. G., Coney, L. R., Zurawski, V. R., Jennings, D. S., & Kamen, B. A. (1992). Cellular localization of the folate receptor: Potential role in drug toxicity and folate homeostasis. Cancer Research, 52(23), 6708-6711.

Cellular localization of the folate receptor : Potential role in drug toxicity and folate homeostasis. / Weitman, Steven D; Weinberg, Arthur G.; Coney, Leslie R.; Zurawski, Vincent R.; Jennings, Debra S.; Kamen, Barton A.

In: Cancer Research, Vol. 52, No. 23, 01.12.1992, p. 6708-6711.

Research output: Contribution to journalArticle

Weitman, SD, Weinberg, AG, Coney, LR, Zurawski, VR, Jennings, DS & Kamen, BA 1992, 'Cellular localization of the folate receptor: Potential role in drug toxicity and folate homeostasis', Cancer Research, vol. 52, no. 23, pp. 6708-6711.
Weitman SD, Weinberg AG, Coney LR, Zurawski VR, Jennings DS, Kamen BA. Cellular localization of the folate receptor: Potential role in drug toxicity and folate homeostasis. Cancer Research. 1992 Dec 1;52(23):6708-6711.
Weitman, Steven D ; Weinberg, Arthur G. ; Coney, Leslie R. ; Zurawski, Vincent R. ; Jennings, Debra S. ; Kamen, Barton A. / Cellular localization of the folate receptor : Potential role in drug toxicity and folate homeostasis. In: Cancer Research. 1992 ; Vol. 52, No. 23. pp. 6708-6711.
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