Cellular characteristics of long-term cultured rat parotid acinar cells

Chih-ko Yeh, P. M. Mertz, C. Oliver, B. J. Baum, E. E. Kousvelari

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

We have successfully maintained and biochemically characterized differentiated rat parotid acinar cells cultured for long periods (6 mo.). The cells were cultured on a reconstituted basement membrane matrix in a medium containing a variety of agents that promote cellular proliferation and differentiation. The cultured cells retain the characteristics of the parental parotid acinar cells. They exhibit both secretory granules and abundant cellular organelles required for protein synthesis and secretion. In situ hybridization and immunocytochemistry demonstrate high levels of proline-rich protein mRNA and protein, and lower levels of amylase mRNA and protein, in their cytoplasm. These findings suggest that rat parotid acinar cells can be maintained in a differentiated state in vitro for long periods, and can serve as a useful model system for studying the regulation of exocrine secretory processes.

Original languageEnglish (US)
Pages (from-to)707-712
Number of pages6
JournalIn Vitro Cellular and Developmental Biology - Animal
Volume27
Issue number9
StatePublished - 1991
Externally publishedYes

Fingerprint

Acinar Cells
Rats
Cultured Cells
Proteins
Messenger RNA
Secretory Pathway
Secretory Vesicles
Amylases
Basement Membrane
Proline
Organelles
In Situ Hybridization
Cytoplasm
Immunohistochemistry
Cells
Cell Proliferation

ASJC Scopus subject areas

  • Developmental Biology
  • Clinical Biochemistry
  • Cell Biology

Cite this

Yeh, C., Mertz, P. M., Oliver, C., Baum, B. J., & Kousvelari, E. E. (1991). Cellular characteristics of long-term cultured rat parotid acinar cells. In Vitro Cellular and Developmental Biology - Animal, 27(9), 707-712.

Cellular characteristics of long-term cultured rat parotid acinar cells. / Yeh, Chih-ko; Mertz, P. M.; Oliver, C.; Baum, B. J.; Kousvelari, E. E.

In: In Vitro Cellular and Developmental Biology - Animal, Vol. 27, No. 9, 1991, p. 707-712.

Research output: Contribution to journalArticle

Yeh, C, Mertz, PM, Oliver, C, Baum, BJ & Kousvelari, EE 1991, 'Cellular characteristics of long-term cultured rat parotid acinar cells', In Vitro Cellular and Developmental Biology - Animal, vol. 27, no. 9, pp. 707-712.
Yeh, Chih-ko ; Mertz, P. M. ; Oliver, C. ; Baum, B. J. ; Kousvelari, E. E. / Cellular characteristics of long-term cultured rat parotid acinar cells. In: In Vitro Cellular and Developmental Biology - Animal. 1991 ; Vol. 27, No. 9. pp. 707-712.
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