Cellular and subcellular localization of 3H-diethylstilboestrol in the central nervous system

Peter J. Sheridan

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

The cellular and subcellular localization of radioactivity in the brain of immature female rats was determined by dry-mount autoradiography 2 h after iv injection of 1.0 μg of (monethyl-3H) diethylstilboestrol per 100 g body weight. A specific topographic pattern of nuclear concentration of the synthetic oestrogen was obtained similar to that for 3H-oestradiol-17 β in specific neurons of the basal hypothalamus, preoptic region and amygdala. In competition experiments, the nuclear concentration of radioactivity in all areas studied was inhibited by unlabeled oestradiol, while unlabeled testosterone had no effect. These data suggest that although oestradiol can bind to androgen receptors, the oestrogen receptor itself can account for the localization seen after the injection of 3H-oestradiol.

Original languageEnglish (US)
Pages (from-to)379-384
Number of pages6
JournalCell and Tissue Research
Volume183
Issue number3
DOIs
StatePublished - Sep 1977

Keywords

  • Autoradiography
  • Brain
  • Diethylstilboestrol
  • Rat

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology

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